BACKGROUND: Diabetes mellitus (DM) has harmful effects on body organs, including submandibular salivary glands (SMGs). It impairs wound healing process that follow sialoadenectomy. Yet there is no complete cure to diabetes, the available medications tend to control the side effects of DM or manage insulin resistance. Herein we tried to investigate the possible effects of injectable platelet rich fibrin (i-PRF) and melatonin on wound healing in diabetic rats. MATERIAL AND METHODS: Surgical defects were created in SMGs of 30 rats after confirmation of DM induction. Then rats were randomly and equally allocated into three groups. Group I served as control group; group II received topically applied i-PRF, and group III received topically administrated melatonin. After 28 days all rats were euthanized, and SMGs were processed for histological and biochemical analysis. RESULTS: Both i-PRF and melatonin caused significant reduction of malondialdehyde (P < 0.0001) and caspase-3 (P < 0.001) and significant increase in vascular endothelial growth factors (P = 0.001,0.009 respectively) that increased SMGs regenerative capacity when compared to diabetic group. Melatonin showed superior results regarding the histomorphological structure of SMGs. CONCLUSION: Melatonin and i-PRF can be possible candidates for improvement of wound healing events in SMGs of diabetic rats.
BACKGROUND: Diabetes mellitus (DM) has harmful effects on body organs, including submandibular salivary glands (SMGs). It impairs wound healing process that follow sialoadenectomy. Yet there is no complete cure to diabetes, the available medications tend to control the side effects of DM or manage insulin resistance. Herein we tried to investigate the possible effects of injectable platelet rich fibrin (i-PRF) and melatonin on wound healing in diabetic rats. MATERIAL AND METHODS: Surgical defects were created in SMGs of 30 rats after confirmation of DM induction. Then rats were randomly and equally allocated into three groups. Group I served as control group; group II received topically applied i-PRF, and group III received topically administrated melatonin. After 28 days all rats were euthanized, and SMGs were processed for histological and biochemical analysis. RESULTS: Both i-PRF and melatonin caused significant reduction of malondialdehyde (P < 0.0001) and caspase-3 (P < 0.001) and significant increase in vascular endothelial growth factors (P = 0.001,0.009 respectively) that increased SMGs regenerative capacity when compared to diabetic group. Melatonin showed superior results regarding the histomorphological structure of SMGs. CONCLUSION: Melatonin and i-PRF can be possible candidates for improvement of wound healing events in SMGs of diabetic rats.
Authors: David M Dohan; Joseph Choukroun; Antoine Diss; Steve L Dohan; Anthony J J Dohan; Jaafar Mouhyi; Bruno Gogly Journal: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Date: 2006-01-10
Authors: Hugo Almeida Varela; Júlio C M Souza; Rubens M Nascimento; Raimundo F Araújo; Roseane C Vasconcelos; Rômulo S Cavalcante; Paulo M Guedes; Aurigena A Araújo Journal: Clin Oral Investig Date: 2018-07-12 Impact factor: 3.573
Authors: Darío Acuña-Castroviejo; Germaine Escames; Carmen Venegas; María E Díaz-Casado; Elena Lima-Cabello; Luis C López; Sergio Rosales-Corral; Dun-Xian Tan; Russel J Reiter Journal: Cell Mol Life Sci Date: 2014-02-20 Impact factor: 9.261
Authors: R J Reiter; S A Rosales-Corral; X Y Liu; D Acuna-Castroviejo; G Escames; D-X Tan Journal: J Periodontal Res Date: 2014-03-25 Impact factor: 4.419
Authors: María Piedad Ramírez-Fernández; José Luis Calvo-Guirado; José Eduardo-Maté Sánchez de-Val; Rafael Arcesio Delgado-Ruiz; Bruno Negri; Guillermo Pardo-Zamora; David Peñarrocha; Cristina Barona; Jose Manuel Granero; Miguel Alcaraz-Baños Journal: Clin Oral Investig Date: 2012-02-11 Impact factor: 3.573