Literature DB >> 329504

[Effect of fixation in Carnoy's solution on nucleic acid and protein concentration in the rabbit superior cervical sympathetic ganglion].

P L Gorelikov.   

Abstract

By means of biochemical techniques, the stability of RNA, DNA and protein contents has been demonstrated in the rabbit superior cervical sympathetic node fixed in Carnoy's solution for 2 hours at 4 degrees C. A 2 hour fixation in Carnoy's fluid at room temperature, results, however, in a considerable loss of RNA (21%). The loss of DNA and protein did not exceed 2% of the total amount of the fresh ganglion tissue. Fixation at a higher temperature (37%) increased the loss percentage protein and of nucleic acids: 3.1, 5.5 and 42%, for protein, DNA and RNA, resp.

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Year:  1977        PMID: 329504

Source DB:  PubMed          Journal:  Tsitologiia        ISSN: 0041-3771


  1 in total

1.  Involvement of N-cholinergic synapses in regulating RNA metabolism in sympathetic neurons and satellite gliocytes.

Authors:  P L Gorelikov; S V Savel'ev
Journal:  Neurosci Behav Physiol       Date:  2008-02
  1 in total

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