| Literature DB >> 32946792 |
Daphne E C Boer1, Mina Mirzaian1, Maria J Ferraz1, Andreea Nadaban2, Anne Schreuder1, Alain Hovnanian3, Jeroen van Smeden4, Joke A Bouwstra2, Johannes M F G Aerts5.
Abstract
The existence of glucosylated cholesterol (GlcChol) in tissue has recently been recognized. GlcChol is generated from glucosylceramide (GlcCer) and cholesterol through transglucosylation by two retaining β-glucosidases, GBA and GBA2. Given the abundance of GBA, GlcCer and cholesterol in the skin's stratum corneum (SC), we studied the occurrence of GlcChol. A significant amount of GlcChol was detected in SC (6 pmol/mg weight). The ratio GlcChol/GlcCer is higher in SC than epidermis, 0.083 and 0.011, respectively. Examination of GlcChol in patients with Netherton syndrome revealed comparable levels (11 pmol/mg). Concluding, GlcChol was identified as a novel component in SC and is likely locally metabolized by GBA. The physiological function of GlcChol in the SC warrants future investigation.Entities:
Keywords: Glucosylated cholesterol; Stratum corneum; β-glucosidase
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Year: 2020 PMID： 32946792 DOI： 10.1016/j.cca.2020.09.017
Source DB: PubMed Journal: Clin Chim Acta ISSN： 0009-8981 Impact factor: 3.786