Literature DB >> 32944644

Adding to the CASeload: unwarranted p53 signaling induced by Cas9.

Veronica Rendo1, Oana M Enache2, Uri Ben-David3.   

Abstract

We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines. For a subset of cell lines with a wild-type tumor protein TP53 (best known as p53), we detected p53 pathway activation, DNA damage accumulation and emerging p53-inactivating mutations following Cas9 introduction. We discuss the potential implications of our findings in basic and translational research.
© 2020 Taylor & Francis Group, LLC.

Entities:  

Keywords:  CRISPR; Cas9; DNA damage response; TP53 mutation; genome editing; p53 pathway

Year:  2020        PMID： 32944644      PMCID： PMC7469564          DOI： 10.1080/23723556.2020.1789419

Source DB:  PubMed          Journal:  Mol Cell Oncol        ISSN： 2372-3556


  8 in total

1.  Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles.

Authors:  Aravind Subramanian; Pablo Tamayo; Vamsi K Mootha; Sayan Mukherjee; Benjamin L Ebert; Michael A Gillette; Amanda Paulovich; Scott L Pomeroy; Todd R Golub; Eric S Lander; Jill P Mesirov
Journal:  Proc Natl Acad Sci U S A       Date:  2005-09-30       Impact factor: 11.205

2.  A Next Generation Connectivity Map: L1000 Platform and the First 1,000,000 Profiles.

Authors:  Aravind Subramanian; Rajiv Narayan; Steven M Corsello; David D Peck; Ted E Natoli; Xiaodong Lu; Joshua Gould; John F Davis; Andrew A Tubelli; Jacob K Asiedu; David L Lahr; Jodi E Hirschman; Zihan Liu; Melanie Donahue; Bina Julian; Mariya Khan; David Wadden; Ian C Smith; Daniel Lam; Arthur Liberzon; Courtney Toder; Mukta Bagul; Marek Orzechowski; Oana M Enache; Federica Piccioni; Sarah A Johnson; Nicholas J Lyons; Alice H Berger; Alykhan F Shamji; Angela N Brooks; Anita Vrcic; Corey Flynn; Jacqueline Rosains; David Y Takeda; Roger Hu; Desiree Davison; Justin Lamb; Kristin Ardlie; Larson Hogstrom; Peyton Greenside; Nathanael S Gray; Paul A Clemons; Serena Silver; Xiaoyun Wu; Wen-Ning Zhao; Willis Read-Button; Xiaohua Wu; Stephen J Haggarty; Lucienne V Ronco; Jesse S Boehm; Stuart L Schreiber; John G Doench; Joshua A Bittker; David E Root; Bang Wong; Todd R Golub
Journal:  Cell       Date:  2017-11-30       Impact factor: 41.582

3.  p53 inhibits CRISPR-Cas9 engineering in human pluripotent stem cells.

Authors:  Robert J Ihry; Kathleen A Worringer; Max R Salick; Elizabeth Frias; Daniel Ho; Kraig Theriault; Sravya Kommineni; Julie Chen; Marie Sondey; Chaoyang Ye; Ranjit Randhawa; Tripti Kulkarni; Zinger Yang; Gregory McAllister; Carsten Russ; John Reece-Hoyes; William Forrester; Gregory R Hoffman; Ricardo Dolmetsch; Ajamete Kaykas
Journal:  Nat Med       Date:  2018-06-11       Impact factor: 53.440

4.  CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response.

Authors:  Emma Haapaniemi; Sandeep Botla; Jenna Persson; Bernhard Schmierer; Jussi Taipale
Journal:  Nat Med       Date:  2018-06-11       Impact factor: 53.440

5.  Cas9 activates the p53 pathway and selects for p53-inactivating mutations.

Authors:  Oana M Enache; Veronica Rendo; Mai Abdusamad; Daniel Lam; Desiree Davison; Sangita Pal; Naomi Currimjee; Julian Hess; Sasha Pantel; Anwesha Nag; Aaron R Thorner; John G Doench; Francisca Vazquez; Rameen Beroukhim; Todd R Golub; Uri Ben-David
Journal:  Nat Genet       Date:  2020-05-18       Impact factor: 38.330

6.  Parallel CRISPR-Cas9 screens clarify impacts of p53 on screen performance.

Authors:  Anne Ramsay Bowden; David A Morales-Juarez; Matylda Sczaniecka-Clift; Maria Martin Agudo; Natalia Lukashchuk; John Christopher Thomas; Stephen P Jackson
Journal:  Elife       Date:  2020-05-22       Impact factor: 8.140

7.  Precise Gene Editing Preserves Hematopoietic Stem Cell Function following Transient p53-Mediated DNA Damage Response.

Authors:  Giulia Schiroli; Anastasia Conti; Samuele Ferrari; Lucrezia Della Volpe; Aurelien Jacob; Luisa Albano; Stefano Beretta; Andrea Calabria; Valentina Vavassori; Patrizia Gasparini; Eralda Salataj; Delphine Ndiaye-Lobry; Chiara Brombin; Julie Chaumeil; Eugenio Montini; Ivan Merelli; Pietro Genovese; Luigi Naldini; Raffaella Di Micco
Journal:  Cell Stem Cell       Date:  2019-03-21       Impact factor: 24.633

8.  Improved estimation of cancer dependencies from large-scale RNAi screens using model-based normalization and data integration.

Authors:  James M McFarland; Zandra V Ho; Guillaume Kugener; Joshua M Dempster; Phillip G Montgomery; Jordan G Bryan; John M Krill-Burger; Thomas M Green; Francisca Vazquez; Jesse S Boehm; Todd R Golub; William C Hahn; David E Root; Aviad Tsherniak
Journal:  Nat Commun       Date:  2018-11-02       Impact factor: 14.919
  8 in total

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