Kaishun Zhao, Chunlin Tu, Kaiyi Liang1, Ying Li2, Yanfang Yu2. 1. Department of Radiology, Jiading Central Hospital, Shanghai University of Medicine & Health Sciences, Shanghai, P. R. China. 2. Department of Respiratory Medicine, Jiading Central Hospital, Shanghai University of Medicine & Health Sciences, Shanghai, P. R. China.
Abstract
OBJECTIVES: This study aimed to investigate the involvement of MCM3AP antisense RNA 1 (MCM3AP-AS1) in chronic obstructive pulmonary disease (COPD). METHODS: The expression levels of plasma MCM3AP-AS1 in COPD patients and healthy controls were measured by quantitative PCR before treatment and at 3 months after the initiation of treatment (post-treatment) from COPD patients. The role of MCM3AP-AS1 in regulating the proliferation of human bronchial smooth muscle cells (HBSMCs) was explored by a cell proliferation assay. RESULTS: We found that MCM3AP-AS1 expression was downregulated in the plasma of COPD patients compared with controls. Among controls, MCM3AP-AS1 expression was lower in smokers than never-smokers. A 3-year follow-up study showed that, among smokers, patients with low MCM3AP-AS1 expression showed a higher incidence of COPD. After treatment for COPD, MCM3AP-AS1 expression significantly increased. The cell proliferation assay showed that MCM3AP-AS1 overexpression decreased the proliferation rate of HBSMCs. MCM3AP-AS1 silencing had the opposite effect. CONCLUSIONS: MCM3AP-AS1 appears to be downregulated in COPD and to predict its occurrence. MCM3AP-AS1 regulates the proliferation of HBSMCs to participate in airway remodeling.
OBJECTIVES: This study aimed to investigate the involvement of MCM3AP antisense RNA 1 (MCM3AP-AS1) in chronic obstructive pulmonary disease (COPD). METHODS: The expression levels of plasma MCM3AP-AS1 in COPDpatients and healthy controls were measured by quantitative PCR before treatment and at 3 months after the initiation of treatment (post-treatment) from COPDpatients. The role of MCM3AP-AS1 in regulating the proliferation of human bronchial smooth muscle cells (HBSMCs) was explored by a cell proliferation assay. RESULTS: We found that MCM3AP-AS1 expression was downregulated in the plasma of COPDpatients compared with controls. Among controls, MCM3AP-AS1 expression was lower in smokers than never-smokers. A 3-year follow-up study showed that, among smokers, patients with low MCM3AP-AS1 expression showed a higher incidence of COPD. After treatment for COPD, MCM3AP-AS1 expression significantly increased. The cell proliferation assay showed that MCM3AP-AS1 overexpression decreased the proliferation rate of HBSMCs. MCM3AP-AS1 silencing had the opposite effect. CONCLUSIONS:MCM3AP-AS1 appears to be downregulated in COPD and to predict its occurrence. MCM3AP-AS1 regulates the proliferation of HBSMCs to participate in airway remodeling.
Authors: D A Mahler; J F Donohue; R A Barbee; M D Goldman; N J Gross; M E Wisniewski; S W Yancey; B A Zakes; K A Rickard; W H Anderson Journal: Chest Date: 1999-04 Impact factor: 9.410
Authors: Amitabh Sharma; Maksim Kitsak; Michael H Cho; Asher Ameli; Xiaobo Zhou; Zhiqiang Jiang; James D Crapo; Terri H Beaty; Jörg Menche; Per S Bakke; Marc Santolini; Edwin K Silverman Journal: Sci Rep Date: 2018-09-27 Impact factor: 4.379
Authors: Natalie Terzikhan; Katia M C Verhamme; Albert Hofman; Bruno H Stricker; Guy G Brusselle; Lies Lahousse Journal: Eur J Epidemiol Date: 2016-03-05 Impact factor: 8.082