| Literature DB >> 32939625 |
Aimee L Thomas1,2, Luke M Evans3,2, Michaela D Nelsen1, Elissa J Chesler4, Matthew S Powers1,2, Winona C Booher1,2, Christopher A Lowry1,5,6,7, John C DeFries8,2, Marissa A Ehringer9,10,11.
Abstract
We conducted whole-genome sequencing of four inbred mouse strains initially selected for high (H1, H2) or low (L1, L2) open-field activity (OFA), and then examined strain distribution patterns for all DNA variants that differed between their BALB/cJ and C57BL/6J parental strains. Next, we assessed genome-wide sharing (3,678,826 variants) both between and within the High and Low Activity strains. Results suggested that about 10% of these DNA variants may be associated with OFA, and clearly demonstrated its polygenic nature. Finally, we conducted bioinformatic analyses of functional genomics data from mouse, rat, and human to refine previously identified quantitative trait loci (QTL) for anxiety-related measures. This combination of sequence analysis and genomic-data integration facilitated refinement of previously intractable QTL findings, and identified possible genes for functional follow-up studies.Entities:
Keywords: Anxiety phenotypes; GeneWeaver; High and Low Activity strains; Quantitative trait loci; Whole-genome sequencing
Mesh：
Year: 2020 PMID： 32939625 PMCID： PMC7854991 DOI： 10.1007/s10519-020-10014-y
Source DB: PubMed Journal: Behav Genet ISSN： 0001-8244 Impact factor: 2.805