Evaluation of the Effectiveness of Screening for Iliac Arterial Calcification in Kidney Transplant Candidates.

BACKGROUND Peripheral vascular disease and iliac arterial calcification are prevalent in kidney transplant candidates and jeopardize graft outcomes. We report our experience with computed tomography (CT) screening for iliac arterial calcification. MATERIAL AND METHODS We retrospectively reviewed electronic medical records of 493 renal transplant candidates from protocol initiation in 2014. Non-contrast CT was performed or retrospectively reviewed if any of the following criteria were present: diabetes, ESRD >6 years, 25 pack-years of smoking or current smoker, diagnosis of peripheral vascular disease, parathyroidectomy, and coronary artery disease intervention. Differences in evaluation and transplant outcomes between groups were compared with chi-squared analysis. Multivariate logistic regression identified predictive criteria for presence of iliac arterial calcification. RESULTS Of 493 candidates evaluated, CTs were reviewed in 346 (70.2%). Iliac arterial calcification was identified in 119 screened candidates (34.4%). Of candidates with iliac arterial calcification identified on CT, 16 (13.4%) were excluded for CT findings, and 9 (7.6%) had their surgical management plan changed. Overall, 91 (76.5%) candidates with iliac arterial calcification on CT were approved, compared to 203 (89.4%) without calcification (P<0.001). The percentage of screened patients with iliac arterial calcification on CT increased with increasing age (P<0.0005). Age and diabetes mellitus were predictive of calcification. CONCLUSIONS Many kidney transplant candidates are at risk for iliac arterial calcification, although such calcification does not prevent transplantation for most candidates who have it. Algorithmic pre-operative screening has clinical value in determining transplant candidacy and potentially improving postoperative outcomes in patients requiring kidney transplantation.

Background

Chronic kidney disease (CKD) affects 1 in 7 adults in the United States, and over 600 000 Americans have end-stage renal disease (ESRD) [1]. Renal transplantation is the treatment of choice for patients with ESRD, and, with the waiting list far exceeding the supply of available organs, the judicious use of organs is imperative [2]. Eligibility for kidney transplant is contingent upon pre-operative evaluation that establishes treatment candidacy and can dictate peri- and postoperative care [3]. Coincident comorbidities can be primary determinants of transplant candidacy and post-transplant outcomes.

Peripheral vascular disease is a common comorbidity found in patients needing kidney transplantation [4] and is characterized by hardening and narrowing of arteries other than in the heart and brain. Peripheral vascular disease can manifest as calcific or occlusive disease [3,5]. Phenotypic plasticity of vascular cells that recapitulates embryonic endochondral ossification is thought to drive vascular calcification by way of bone morphogenetic proteins that are triggered and modulated by a number of inflammatory, metabolic, and genetic disorders [6,7]. Vascular calcification is very common in CKD patients; in fact, the most extensive vascular calcification occurs in patients with CKD [6].

Although peripheral vascular disease is very prevalent among patients with ESRD, it is often underdiagnosed in this patient population [4]. However, its identification in transplant patients is essential because of the known adverse relationship between peripheral vascular disease and transplant outcomes. Lower extremity peripheral arterial disease is associated with lower patient survival after renal transplantation, and moderate-to-severe lower extremity arterial calcification is associated with increased waitlist mortality and lower patient and graft survival after transplantation [4,8,9]. Peripheral vascular disease is also one of the core parameters utilized in preoperative risk stratification indexes for mortality prediction in transplant candidates [10]. It has also been associated with significant coronary vascular disease requiring coronary intervention prior to renal transplantation [11]. Surgically, peripheral vascular disease of the iliac arteries jeopardizes patient outcomes owing to either compromised inflow to the transplanted kidney or implantation failure due to vessel calcification. While occlusive disease can be identified clinically, calcific disease is often silent. There is currently no consensus on the most appropriate technique to diagnose vascular calcification in patients with CKD [12,13].

We recently implemented a computed tomography (CT) scan screening protocol for the detection and characterization of iliac arterial calcification in patients with CKD. To better understand this patient population, we assessed the pre-screening attributes of an internal cohort of patients before and after implementation of the screening protocol. We characterized post-screening clinical decision making from a systems level perspective and further identified factors within the established pre-screening parameters that were significantly associated with a positive CT scan for iliac arterial calcification. The aims of this study are to inform similarly sized health systems of the effectiveness of preoperative screening and to identify the subset of patients most likely to benefit from such screening.

Material and Methods

This was a single-center retrospective observational study of patients evaluated for renal transplantation. We retrospectively reviewed the electronic medical records of 1092 renal allograft candidates from 2007 to 2015 at the University of Michigan Health System. Starting in 2014, transplant candidates, including those already listed or previously evaluated, were screened for iliac arterial calcification by non-contrast CT scan. Institutional Review Board approval was obtained, and the study was exempt for approval from an ethics board. Those with signs and symptoms of occlusive disease received contrast CT where indicated. While venous disease could be identified in the CT scan assessment, the protocol was specifically designed to address peripheral arterial disease, and more specifically calcification.

The screening criteria included: 1) history or symptoms of occlusive disease: claudication, rest pain, presence of non-healing ulcer(s), diminished femoral pulses, or calcified vessels on physical exam; 2) ESRD for more than 6 years; 3) currently smoking or history of more than 25 pack-years; 4) diabetes mellitus; 5) intervention for coronary artery disease; and 6) parathyroidectomy (hyperparathyroidism is a risk factor for peripheral vascular calcification) [14].

Patients who qualified for screening based on the above criteria underwent a non-contrast CT scan to identify iliac artery calcifications. All CTs were reviewed by a transplant surgeon. Degree of calcification was defined as: no disease: calcification in the iliac arteries is absent or minimal; moderate disease: calcification is limited to the common iliac artery or, if present in the external iliac artery, does not affect ability to occlude the artery or perform anastomosis; and severe disease: calcification is significant enough to anticipate requiring either anastomosis or clamping in calcified areas, or prohibits transplant altogether.

Candidates could have been excluded based on CT findings alone, or in consideration of overall risk. Patients with potentially significant iliac occlusive disease were either worked up further with noninvasive flow studies or referred to a vascular surgeon.

We identified 493 patients who met the above criteria and were screened using the new protocol; reasons for screening were recorded (Figure 1). Patients were excluded if they had previous transplants, did not meet the screening criteria, or had an incomplete medical record. Of the total patients, 346 had CT scans evaluated for iliac arterial calcification and were classified based on severity or extent of calcification on CT, as described above. Data were recorded on transplant acceptance and whether non-acceptance was attributable to CT findings. The primary outcome measures were identification of calcification and the ability of the screening protocol to predict calcification. Secondary outcomes were the impact of CT findings on either transplant candidacy or operative approach (i.e., whether the CT findings directed either laterality of the recipient operation or specified the target artery).

Figure 1

Study population, exclusions, outcomes of CT scanning, and candidate acceptance.

Differences between groups were compared with chi-squared analysis for categorical variables. Multivariate logistic regression analysis was conducted to identify significant associations between the eligibility criteria and the identification of moderate or severe disease.

Results

Table 1 shows the number of patients that matched each criterion, as well as the number of patients screened, their screening outcomes, and whether management was changed as a result of CT findings. Diabetes was the most common criterion in candidates who qualified for screening and in those screened. However, iliac arterial calcification was most frequently identified in those candidates who had smoking and coronary artery disease intervention as screening criteria, and these patients were most frequently ruled out owing to severity of disease. Table 2 highlights the demographics of this cohort.

Table 1

Analysis of evaluated patients based on six screening criteria.

Evaluation criteria met	Total patients	Evaluated with CT	Evaluated with CT and calcification found	Evaluated with CT and ruled out for any reason	Evaluated with CT and ruled out due to calcification	Management change
Current Smoker or Smoking >25 years	112 (22.7%)	70 (62.5%)	36 (32.1%)	16 (14.3%)	7 (6.3%)	2 (1.8%)
Diabetes	360 (72.9%)	270 (75%)	98 (27.2%)	38 (10.6%)	14 (3.9%)	9 (2.5%)
ESRD >6 years	124 (25.1%)	69 (55.6%)	22 (17.7%)	9 (7.3%)	1 (0.8%)	1 (0.8%)
History PAD	164 (33.2%)	113 (68.9%)	46 (28.0%)	22 (13.4%)	7 (4.3%)	3 (1.8%)
CAD Intervention	149 (30.2%)	104 (69.8%)	51 (34.2%)	22 (14.8%)	10 (6.7%)	3 (2.0%)
Parathyroid-ectomy	21 (4.3%)	14 (66.7%)	3 (14.3%)	1 (4.8%)	0 (0.0%)	0 (0.0%)
Total	493	346 (70.2%)	119 (24.1%)	51 (10.3%)	16 (3.2%)	9 (1.8%)

Table 2

Patient characteristics.

Patient characteristics	n=346
Mean Age	60
Gender
Male	213 (61%)
Female	133 (38%)
Race
American Indian and Alaska Native	2 (0.6%)
Asian	6 (1.7%)
Black or African American	85 (24.6%)
Native Hawaiian and other Pacific Islander	1 (0.3%)
Other	19 (5.5%)
Unknown	6 (1.7%)
White or Caucasian	227 (65.6%)
History of prior transplant	66 (19.0%)

Of the patients that qualified for screening, 379 (nearly 80%) met only 1 or 2 of the criteria, and a small proportion met several (Figure 2). Of the 346 patients screened with a CT scan, 119 patients (34.4%) were found to have iliac arterial calcification on CT; 77 had moderate disease; and 42 had severe disease. These patients were divided into age groups (Figure 3), and a significant association (P<0.0005) was found between increasing age and identification of calcification. No patients under the age of 35 were found to have iliac arterial calcification on CT.

Figure 2

The patient cohort was analyzed to determine how many of the 6 screening criteria each patient met. The number of patients and percentage of the entire cohort meeting 1 through 6 of the criteria is shown. The majority of patients met 1 or 2 of the criteria.

Figure 3

Age distribution of patients that were found to have iliac arterial calcification on CT scan.

Of the 119 patients with iliac arterial calcification, 91 (76.5%) were accepted for transplant listing, compared to the 203 of 227 patients (89.4%) without calcification that were accepted (P<0.001, Table 3). Of the 51 patients ruled out after evaluation with a CT scan, 16 (31.4%) were ruled out exclusively owing to severe iliac arterial calcification. The remaining 35 patients (68.6%) were ruled out for severe iliac arterial calcification in addition to other reasons, or for other reasons entirely. We further classified patients by the extent of iliac arterial calcification found on CT (Figure 4) and found that patients with severe but not moderate iliac arterial calcification on CT were also twice as likely than those with no calcification to get ruled out for other reasons, though this difference was not significant (P=0.39). Among the patients that were accepted for transplant, CT findings directed surgical management in 9 patients (2.6%) either by influencing the choice of side (left vs. right) or by altering the target artery to the common iliac artery.

Table 3

Impact of finding iliac arterial calcification on CT scan on listing decision.

CT Finding	Ruled out	Accepted	Total	% Accepted
Calcification on CT	28	91	119	76.5%
No Calcification on CT	23	203	227	89.4%
Significance				p<0.001

Figure 4

Patients evaluated with CT scan were categorized by the extent of iliac arterial calcification found on CT: no, moderate, or severe disease. Each group was further analyzed to determine the percentage of patients who were ruled out for other or additional reasons. Patients who were ruled out exclusively on the basis of arterial calcification were excluded from this analysis.

Looking further into each screening criterion, we found that no patients were ruled out owing to iliac arterial calcification on CT when the only criterion they met was either ESRD >6 years or parathyroidectomy. Among the 124 patients with ESRD >6 years, 38 met no other screening criteria, and among this subgroup no patients were ruled out because of iliac arterial calcification. Among the 21 patients with a history of a parathyroidectomy, 5 met no other screening criteria and none were ruled out because of iliac arterial calcification in this subgroup. All other screening criteria resulted in the unique identification of calcification that resulted in patients being ruled out for transplant candidacy.

We conducted multivariate regression analysis to identify the screening criteria that were most likely to be associated with iliac arterial calcification on CT. Gender, age, race, and BMI were included with the protocol screening criteria in the full model. Age and diabetes were significantly associated with iliac arterial calcification on CT, corroborating earlier findings that older patients in this screened population were more likely to exhibit iliac arterial calcification on CT (Table 4).

Table 4

Multivariate regression analysis.

	Odds ratio (95% CI)	p-Value
Sex	0.88 (0.54 to 1.41)	0.077
Age*	1.03 (1.01 to 1.06)	9.7×10−5
BMI	0.97 (0.93 to 1.01)	0.18
Current smoker or >25 year smoker	1.59 (0.94 to 2.65)	0.14
Diabetes mellitus*	2.33 (1.31 to 4.27)	0.0034
ESRD >6 years	1.10 (0.60 to 1.98)	0.81
History of PAD	1.22 (0.76 to 1.95)	0.23
CAD intervention	1.56 (0.96 to 2.50)	0.070
Parathyroidectomy	0.89 (0.19 to 2.98)	0.86

Discussion

Ensuring the long-term success of renal transplantation is critical to maintaining the capacity of health systems to manage the increasing burden of ESRD. Given the rising demand for organ transplantation, predictive algorithms that can identify candidates with a lower likelihood of developing short- and long-term complications will be instrumental in facilitating the judicious allocation of resources. Iliac arterial calcification is important because it can negatively affect transplant outcome. Imaging modalities such as CT and X-ray that screen for vascular calcification have an essential role in informing mortality risk and therapeutic approaches in patients with CKD [15]. Here, we report the implementation of a novel screening protocol that leverages CT scans to identify calcific/occlusive disease in high-risk renal transplant candidates.

As is common with new protocols, we had a transition period before full implementation. As such, of the 493 patients that qualified for the screening protocol, 346 (70.2%) underwent CT scanning. For those who underwent screening, our results showed concordance between the extent of calcific disease found on CT scan and the proportion of patients who were ruled out not only for this disease but for any cause. Intriguingly, a majority of these patients were ruled out for reasons other than calcific disease, demonstrating either that iliac calcification on CT acted as a surrogate for poor transplant candidacy or that the additional risk of calcification was a contributory factor in otherwise marginal candidates. Furthermore, the protocol identified 16 patients with calcific disease that was too extensive to be considered for transplantation listing regardless of other risks.

Among the 6 screening criteria, the magnitude of associations with iliac arterial calcification identification varied. Logistic regression analysis demonstrated that, of the 6 criteria, only diabetic status was significantly associated with calcification. To understand the importance of each criterion’s associations with calcification, we isolated each criterion and looked at the patients that qualified for screening based on that criterion alone. We found that among patients qualifying because of either ESRD >6 years or history of parathyroidectomy alone, no patients were ruled out because of iliac arterial calcification. Hence, despite evidence that suggested increased risk of arterial calcification in these patients, we did not find this to be indicative of iliac calcification in our population, and thus we removed these criteria from the screening protocol.

Age was highly associated with arterial calcification in this study, which is consistent with prior work showing that the prevalence of coronary calcification rises with advancing age [4]. While age is a known risk factor for peripheral arterial disease and coronary calcification, it is important to remember that this is a screened population with other risk factors for calcification (diabetes, coronary artery disease, smoking, ESRD vintage), and age is not necessarily a priori associated with clinically significant iliac arterial calcification, as defined by this study in this population. Indeed, our experience with CT reviews performed outside of this protocol in candidates who did not meet screening criteria suggest that age alone should not be included in the absence of other risk factors. However, our results do suggest that screening is not necessary in any candidate under age 35, regardless of other risks, as no patient under age 35 was found to have iliac arterial calcification on CT.

Of the patients screened for iliac arterial calcification using CT scanning, 9 patients (2.6%) underwent a change in transplant laterality or in the vessel used for transplant owing to findings in the CT scan. This demonstrates that the protocol, in addition to finding high-risk candidates, can be used to better individualize surgical approach.

While our screening protocol appears to have measurable benefits, several limitations impacted the conclusions of our study. Many patients were not included in the analyses because of incomplete medical history. Not all patients that met criteria were imaged, which is a logistical consideration to be further evaluated and addressed. The scale used to classify the extent of calcification on the CT scans was a subjective scale rather than an objective scale. Operator dependency is a widely accepted source of inconsistency when evaluating radiographic findings, and having varying physicians label the extent of calcification may have introduced heterogeneity into the results. Therefore, our findings may have limited statistical power owing to exclusion, incomplete screening implementation, and operator inconsistency in the determination of the extent of calcific disease.

The number of patients that have undergone the screening protocol and gone on to be transplanted is low given the recent implementation of the protocol, and thus additional time and patient volume will improve the power of our analyses. Above and beyond determining surgical candidacy, the utilization of CT indicators of the overall burden of calcification may further inform outcomes, given the previously reported associations with cardiovascular events and mortality in dialysis patients [12,16].

Conclusions

The impact of iliac arterial calcification on renal transplantation is significant. Our findings after the initial implementation of a novel protocol utilizing CT scanning for screening of iliac arterial calcification show promise to improve transplant patient selection. The protocol helped to rule out candidates with extensive disease or identified patients that were ruled out for additional reasons. Therefore, the protocol successfully helped identify less suitable transplant candidates with higher risk of adverse outcomes and complications, many of whom may have experienced an aborted transplant operation in the absence of screening. The sub-analysis of each screening criteria identified possible enhancements to the protocol, namely, to exclude patients under the age of 35, and to include only 4 criteria: history of diabetes mellitus, smoking status, history of coronary artery disease intervention, and history of peripheral artery disease. Though our preliminary findings support the continued use of the protocol, longitudinal data is needed to determine whether the protocol enhances transplant outcomes and if it effectively identifies patients at risk for inferior outcomes.