Julien Marlet1, Camille Petillon2, Emma Ragot2, Yazid Abou El Fattah2, Antoine Guillon3, Sylvain Marchand Adam4, Adrien Lemaignen5, Louis Bernard5, Guillaume Desoubeaux6, Hélène Blasco7, Francis Barin8, Karl Stefic8, Catherine Gaudy-Graffin8. 1. Service de Bactériologie-Virologie-Hygiène, CHRU de Tours, France; INSERM U1259, Université de Tours, France. Electronic address: julien.marlet@univ-tours.fr. 2. Service de Bactériologie-Virologie-Hygiène, CHRU de Tours, France. 3. Service de Réanimation Polyvalente, CHRU de Tours, France; Inserm, U1100, Centre d'Etude des Pathologies Respiratoires, Université de Tours, Tours, France. 4. Service de Pneumologie et Explorations Fonctionnelles Respiratoires, CHRU de Tours, France. 5. Service de Médecine Interne et Maladies Infectieuses, CHRU de Tours, France. 6. Service de Parasitologie et Mycologie - Médecine tropicale, CHRU de Tours, France. 7. Service de Biochimie et Biologie Moléculaire, CHRU de Tours, France. 8. Service de Bactériologie-Virologie-Hygiène, CHRU de Tours, France; INSERM U1259, Université de Tours, France.
Abstract
OBJECTIVES: The aim of the present study was to evaluate the clinical performance of four SARS-CoV-2 immunoassays and their contribution in routine care for the diagnosis of COVID-19, in order to benefit of robust data before their extensive use. METHODS: The clinical performance of Euroimmun ELISA SARS-CoV-2 IgG, Abbott SARS-CoV-2 IgG, Wantai SARS-CoV-2 Ab ELISA, and DiaPro COVID-19 IgG confirmation were evaluated in the context of both a retrospective and a prospective analysis of COVID-19 patients. The retrospective analysis included plasma samples from 63 COVID-19 patients and 89 control (pre-pandemic) patients. The prospective study included 203 patients who tested either negative (n = 181) or positive (n = 22) by RT-PCR before serology sampling. RESULTS: The specificity was 92.1 %, 98.9 %, 100 % and 98.9 % and the sensitivity 14 days after onset of symptoms was 95.6 %, 95.6 %, 97.8 % and 95.6 % for Euroimmun IgG, Abbott IgG, Wantai Ab, and DiaPro IgG confirmation SARS-CoV-2 immunoassays, respectively. The low specificity of Euroimmun IgG (for ratio <5) was not confirmed in routine care setting (98.5 % negative agreement). Serology was complementary to RT-PCR in routine care and lead to identification of false positive (Ct>38, <2 targets detected) and false negative RT-PCR results (>1 month post onset of symptoms). CONCLUSIONS: Serology was complementary to RT-PCR for the diagnosis of COVID-19 at least 14 days after onset of symptoms. First line serology testing can be performed with Wantai Ab or Abbott IgG assays, while DiaPro IgG confirmation assay can be used as an efficient confirmation assay.
OBJECTIVES: The aim of the present study was to evaluate the clinical performance of four SARS-CoV-2 immunoassays and their contribution in routine care for the diagnosis of COVID-19, in order to benefit of robust data before their extensive use. METHODS: The clinical performance of Euroimmun ELISA SARS-CoV-2 IgG, Abbott SARS-CoV-2 IgG, Wantai SARS-CoV-2 Ab ELISA, and DiaProCOVID-19 IgG confirmation were evaluated in the context of both a retrospective and a prospective analysis of COVID-19patients. The retrospective analysis included plasma samples from 63 COVID-19patients and 89 control (pre-pandemic) patients. The prospective study included 203 patients who tested either negative (n = 181) or positive (n = 22) by RT-PCR before serology sampling. RESULTS: The specificity was 92.1 %, 98.9 %, 100 % and 98.9 % and the sensitivity 14 days after onset of symptoms was 95.6 %, 95.6 %, 97.8 % and 95.6 % for Euroimmun IgG, Abbott IgG, Wantai Ab, and DiaPro IgG confirmation SARS-CoV-2 immunoassays, respectively. The low specificity of Euroimmun IgG (for ratio <5) was not confirmed in routine care setting (98.5 % negative agreement). Serology was complementary to RT-PCR in routine care and lead to identification of false positive (Ct>38, <2 targets detected) and false negative RT-PCR results (>1 month post onset of symptoms). CONCLUSIONS: Serology was complementary to RT-PCR for the diagnosis of COVID-19 at least 14 days after onset of symptoms. First line serology testing can be performed with Wantai Ab or Abbott IgG assays, while DiaPro IgG confirmation assay can be used as an efficient confirmation assay.
Authors: N Davidson; J Evans; D Giammichele; H Powell; P Hobson; B Teis; H Glover; K B Guppy-Coles; J Robson Journal: Pathology Date: 2020-09-30 Impact factor: 5.306
Authors: Miroslav Fajfr; Radek Sleha; Sylva Janovska; Vladimir Koblizek; Mikulas Skala; Stanislav Plisek; Petr Prasil; Petr Smahel; Pavel Bostik Journal: Viruses Date: 2022-03-04 Impact factor: 5.048