Anna-Lena Feder1, Eric Pion1, Johannes Troebs1, Ulrich Lenze2, Lukas Prantl3, Maung Mg Htwe4, Aung Phyo4, Silke Haerteis1, Thiha Aung1,3,4. 1. Institute for Molecular and Cellular Anatomy, University of Regensburg, Regensburg, Germany. 2. Department of Orthopaedics and Sportorthopaedics, Technical University of Munich, Klinikum rechts der Isar, Munich, Germany. 3. Center of Plastic, Aesthetic, Hand and Reconstructive Surgery, University of Regensburg, Regensburg, Germany. 4. Sarcoma and Musculoskeletal Oncoplastic Division, Department of Orthopaedic Surgery, University of Medicine, Mandalay, Myanmar.
Abstract
BACKGROUND: Osteosarcomas are a rare, heterogeneous and malignant group of bone tumors that have a high potential for metastasis and aggressive growth patterns. Treatment of metastasized osteosarcoma is often insufficient and research is compromised by problems encountered when culturing cells or analyzing genetic alterations due to the high level of intratumoral and intertumoral heterogeneity. The chick chorioallantoic membrane (CAM) model, a 3D-in-vivo-tumor-model, could potentially facilitate the investigation of osteosarcoma heterogeneity at an individual and highly specified level. OBJECTIVE: Objective was to establish the grafting and transplantation of different primary osteosarcoma tissue parts onto several consecutive CAMs for tumor profiling and investigation of osteosarcoma heterogeneity. METHODS: Various parts of primary osteosarcoma tissue were grafted onto CAMs and were transplanted onto another CAM for five to seven consecutive times, enabling further experimental analyzes. RESULTS: Primary osteosarcoma tissue parts exhibited satisfactory growth patterns and displayed angiogenic development on the CAM. It was possible to graft and transplant different tumor parts several times while the tissue viability was still high and tumor profiling was performed. CONCLUSIONS: Primary osteosarcoma tissue grew on several different CAMs for an extended time period and neovascularization of serial transplanted tumor parts was observed, improving the versatility of the 3D-in-vivo-tumor-model.
BACKGROUND:Osteosarcomas are a rare, heterogeneous and malignant group of bone tumors that have a high potential for metastasis and aggressive growth patterns. Treatment of metastasized osteosarcoma is often insufficient and research is compromised by problems encountered when culturing cells or analyzing genetic alterations due to the high level of intratumoral and intertumoral heterogeneity. The chick chorioallantoic membrane (CAM) model, a 3D-in-vivo-tumor-model, could potentially facilitate the investigation of osteosarcoma heterogeneity at an individual and highly specified level. OBJECTIVE: Objective was to establish the grafting and transplantation of different primary osteosarcoma tissue parts onto several consecutive CAMs for tumor profiling and investigation of osteosarcoma heterogeneity. METHODS: Various parts of primary osteosarcoma tissue were grafted onto CAMs and were transplanted onto another CAM for five to seven consecutive times, enabling further experimental analyzes. RESULTS:Primary osteosarcoma tissue parts exhibited satisfactory growth patterns and displayed angiogenic development on the CAM. It was possible to graft and transplant different tumor parts several times while the tissue viability was still high and tumor profiling was performed. CONCLUSIONS:Primary osteosarcoma tissue grew on several different CAMs for an extended time period and neovascularization of serial transplanted tumor parts was observed, improving the versatility of the 3D-in-vivo-tumor-model.
Authors: Konstantin Drexler; Katharina M Schmidt; Katrin Jordan; Marianne Federlin; Vladimir M Milenkovic; Gerhard Liebisch; Anna Artati; Christian Schmidl; Gregor Madej; Janina Tokarz; Alexander Cecil; Wolfgang Jagla; Silke Haerteis; Thiha Aung; Christine Wagner; Maria Kolodziejczyk; Stefanie Heinke; Evan H Stanton; Barbara Schwertner; Dania Riegel; Christian H Wetzel; Wolfgang Buchalla; Martin Proescholdt; Christoph A Klein; Mark Berneburg; Hans J Schlitt; Thomas Brabletz; Christine Ziegler; Eric K Parkinson; Andreas Gaumann; Edward K Geissler; Jerzy Adamski; Sebastian Haferkamp; Maria E Mycielska Journal: Life Sci Alliance Date: 2021-03-23
Authors: Eva-Marie Bichlmayer; Lina Mahl; Leo Hesse; Eric Pion; Victoria Haller; Andreas Moehwald; Christina Hackl; Jens M Werner; Hans J Schlitt; Siegfried Schwarz; Philipp Kainz; Christoph Brochhausen; Christian Groeger; Felix Steger; Oliver Kölbl; Christoph Daniel; Kerstin Amann; Andre Kraus; Björn Buchholz; Thiha Aung; Silke Haerteis Journal: Cells Date: 2022-07-22 Impact factor: 7.666