Literature DB >> 3291877

The cloning and over-expression of PABA synthase in E. coli.

M J McLeish1, P J Wookey, K G Mortimer.   

Abstract

Both the genes encoding E. coli p-aminobenzoic acid synthase have been cloned and an overproducing strain has been obtained. The partial purification of the large subunit is described. The kinetic properties of the cloned enzyme, while similar to those reported for the B. subtilis enzyme, show some differences to those reported for the S. griseus enzyme.

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Year:  1988        PMID: 3291877

Source DB:  PubMed          Journal:  Biochem Int        ISSN: 0158-5231


  2 in total

1.  p-Aminobenzoate synthesis in Escherichia coli: purification and characterization of PabB as aminodeoxychorismate synthase and enzyme X as aminodeoxychorismate lyase.

Authors:  Q Z Ye; J Liu; C T Walsh
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

2.  Kinetic characterization of 4-amino 4-deoxychorismate synthase from Escherichia coli.

Authors:  V K Viswanathan; J M Green; B P Nichols
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490

  2 in total

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