Fernanda Cristina Carvalho Mattos Magno1,2, Helena Chrispim Guaraná3, Ana Carolina Proença da Fonseca4,5, Aline Pereira Pedrosa3, Verônica Marques Zembrzuski4, Pedro Hernan Cabello4,6, Giselda Maria Kalil Cabello4, João Régis Ivar Carneiro7, Eliane Lopes Rosado3. 1. Institute of Nutrition, Federal University of Rio de Janeiro, Carlos Chagas Filho Avenue, 373, CCS, Block J, 2nd Floor, University City, Fundão Island, Rio de Janeiro, RJ, 21941-590, Brazil. fernandamattos.nut@gmail.com. 2. Department of Nutrology, Federal University of Rio de Janeiro, University Hospital Clementino Fraga Filho, Professor Rodolpho Paulo Rocco Street, 255, 9th Floor, University City, Fundão Island, Rio de Janeiro, RJ, 21941-913, Brazil. fernandamattos.nut@gmail.com. 3. Institute of Nutrition, Federal University of Rio de Janeiro, Carlos Chagas Filho Avenue, 373, CCS, Block J, 2nd Floor, University City, Fundão Island, Rio de Janeiro, RJ, 21941-590, Brazil. 4. Oswaldo Cruz Foundation (FIOCRUZ), Oswaldo Cruz Institute (IOC), Brazil Avenue, 4365, Leônidas Deane Building, 6th Floor, Human Genetics Laboratory, Room 615, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil. 5. Oswaldo Cruz Foundation (FIOCRUZ), Oswaldo Cruz Institute (IOC), Brazil Avenue, 4365, Pavilion 108, Laboratory of Immunopharmacology, Room 44, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil. 6. Human Genetics Laboratory, Professor José de Souza Herdy Street, 1160, Jardim Vinte E Cinco de Agosto, Duque de Caxias, RJ, 25071-202, Brazil. 7. Department of Nutrology, Federal University of Rio de Janeiro, University Hospital Clementino Fraga Filho, Professor Rodolpho Paulo Rocco Street, 255, 9th Floor, University City, Fundão Island, Rio de Janeiro, RJ, 21941-913, Brazil.
Abstract
PURPOSE: The rs17782313 variant of the MC4R gene plays an important role in the obesity phenotype. Studies that evaluate environmental factors and genetic variants associated with obesity may represent a great advance in understanding the development of this disease. This work seeks to assess the association of the polymorphism of MC4R rs17782313 on plasma parameters, including leptin, ghrelin, tumor necrosis factor (TNFα) and interleukin 6 (IL6), and on the eating behaviors of morbidly obese women. METHODS: 70 adult women with BMI between 40 and 60 kg/m2 were recruited. Laboratory and anthropometric data were recorded. Using a visual analog scale (VAS), the feelings of hunger and satiety were evaluated. The presence or absence of binge eating was evaluated through the Binge Eating Scale (BES) questionnaire. Habitual food intake was analyzed using 3-day dietary records. TaqMan® assays were conducted using real-time PCR to assess genotype polymorphism variants from peripheral blood DNA. RESULTS: This study found that female patients with the MC4R rs17782313 polymorphism had high levels of ghrelin and reduced levels of IL6 in the postprandial period. We observed a higher prevalence of severe binge eating in more than 50% of women with at least one risk allele. CONCLUSION: Our hypothesis is that the MC4R rs17782313 polymorphism may influence the release of ghrelin, even without being associated with feelings of hunger and satiety. More than half of women with this polymorphism exhibited severe binge eating. LEVEL OF EVIDENCE: Level III: case-control analytic study.
PURPOSE: The rs17782313 variant of the MC4R gene plays an important role in the obesity phenotype. Studies that evaluate environmental factors and genetic variants associated with obesity may represent a great advance in understanding the development of this disease. This work seeks to assess the association of the polymorphism of MC4R rs17782313 on plasma parameters, including leptin, ghrelin, tumor necrosis factor (TNFα) and interleukin 6 (IL6), and on the eating behaviors of morbidly obese women. METHODS: 70 adult women with BMI between 40 and 60 kg/m2 were recruited. Laboratory and anthropometric data were recorded. Using a visual analog scale (VAS), the feelings of hunger and satiety were evaluated. The presence or absence of binge eating was evaluated through the Binge Eating Scale (BES) questionnaire. Habitual food intake was analyzed using 3-day dietary records. TaqMan® assays were conducted using real-time PCR to assess genotype polymorphism variants from peripheral blood DNA. RESULTS: This study found that female patients with the MC4R rs17782313 polymorphism had high levels of ghrelin and reduced levels of IL6 in the postprandial period. We observed a higher prevalence of severe binge eating in more than 50% of women with at least one risk allele. CONCLUSION: Our hypothesis is that the MC4R rs17782313 polymorphism may influence the release of ghrelin, even without being associated with feelings of hunger and satiety. More than half of women with this polymorphism exhibited severe binge eating. LEVEL OF EVIDENCE: Level III: case-control analytic study.
Authors: D Huszar; C A Lynch; V Fairchild-Huntress; J H Dunmore; Q Fang; L R Berkemeier; W Gu; R A Kesterson; B A Boston; R D Cone; F J Smith; L A Campfield; P Burn; F Lee Journal: Cell Date: 1997-01-10 Impact factor: 41.582
Authors: Otto Tschritter; Axel Haupt; Hubert Preissl; Caroline Ketterer; Anita M Hennige; Tina Sartorius; Fausto Machicao; Andreas Fritsche; Hans-Ulrich Häring Journal: J Obes Date: 2011-06-03
Authors: Ruth J F Loos; Cecilia M Lindgren; Shengxu Li; Eleanor Wheeler; Jing Hua Zhao; Inga Prokopenko; Michael Inouye; Rachel M Freathy; Antony P Attwood; Jacques S Beckmann; Sonja I Berndt; Kevin B Jacobs; Stephen J Chanock; Richard B Hayes; Sven Bergmann; Amanda J Bennett; Sheila A Bingham; Murielle Bochud; Morris Brown; Stéphane Cauchi; John M Connell; Cyrus Cooper; George Davey Smith; Ian Day; Christian Dina; Subhajyoti De; Emmanouil T Dermitzakis; Alex S F Doney; Katherine S Elliott; Paul Elliott; David M Evans; I Sadaf Farooqi; Philippe Froguel; Jilur Ghori; Christopher J Groves; Rhian Gwilliam; David Hadley; Alistair S Hall; Andrew T Hattersley; Johannes Hebebrand; Iris M Heid; Claudia Lamina; Christian Gieger; Thomas Illig; Thomas Meitinger; H-Erich Wichmann; Blanca Herrera; Anke Hinney; Sarah E Hunt; Marjo-Riitta Jarvelin; Toby Johnson; Jennifer D M Jolley; Fredrik Karpe; Andrew Keniry; Kay-Tee Khaw; Robert N Luben; Massimo Mangino; Jonathan Marchini; Wendy L McArdle; Ralph McGinnis; David Meyre; Patricia B Munroe; Andrew D Morris; Andrew R Ness; Matthew J Neville; Alexandra C Nica; Ken K Ong; Stephen O'Rahilly; Katharine R Owen; Colin N A Palmer; Konstantinos Papadakis; Simon Potter; Anneli Pouta; Lu Qi; Joshua C Randall; Nigel W Rayner; Susan M Ring; Manjinder S Sandhu; André Scherag; Matthew A Sims; Kijoung Song; Nicole Soranzo; Elizabeth K Speliotes; Holly E Syddall; Sarah A Teichmann; Nicholas J Timpson; Jonathan H Tobias; Manuela Uda; Carla I Ganz Vogel; Chris Wallace; Dawn M Waterworth; Michael N Weedon; Cristen J Willer; Xin Yuan; Eleftheria Zeggini; Joel N Hirschhorn; David P Strachan; Willem H Ouwehand; Mark J Caulfield; Nilesh J Samani; Timothy M Frayling; Peter Vollenweider; Gerard Waeber; Vincent Mooser; Panos Deloukas; Mark I McCarthy; Nicholas J Wareham; Inês Barroso; Kevin B Jacobs; Stephen J Chanock; Richard B Hayes; Claudia Lamina; Christian Gieger; Thomas Illig; Thomas Meitinger; H-Erich Wichmann; Peter Kraft; Susan E Hankinson; David J Hunter; Frank B Hu; Helen N Lyon; Benjamin F Voight; Martin Ridderstrale; Leif Groop; Paul Scheet; Serena Sanna; Goncalo R Abecasis; Giuseppe Albai; Ramaiah Nagaraja; David Schlessinger; Anne U Jackson; Jaakko Tuomilehto; Francis S Collins; Michael Boehnke; Karen L Mohlke Journal: Nat Genet Date: 2008-05-04 Impact factor: 38.330
Authors: Alastair S Garfield; Chia Li; Joseph C Madara; Bhavik P Shah; Emily Webber; Jennifer S Steger; John N Campbell; Oksana Gavrilova; Charlotte E Lee; David P Olson; Joel K Elmquist; Bakhos A Tannous; Michael J Krashes; Bradford B Lowell Journal: Nat Neurosci Date: 2015-04-27 Impact factor: 24.884