Chetna Gangwar1, Suresh Dinkar Kharche2, Anil Kumar Mishra3, Sonia Saraswat4, Narendra Kumar5, Anuj Kumar Sikarwar4. 1. Animal Reproduction, AP&R Division, ICAR- Central Institute for Research on Goats, Makhdoom, Farah, Mathura, 281122, India. chetnaom82@gmail.com. 2. Animal Reproduction, AP&R Division, ICAR- Central Institute for Research on Goats, Makhdoom, Farah, Mathura, 281122, India. 3. Veterinary Microbiology, Animal Health Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, 281122, India. 4. Animal Biotechnology, AP&R Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, 281122, India. 5. LPM, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura, 281122, India.
Abstract
OBJECTIVE: The aim of the study was to explore the possibility of a better sugar suitable for storage of goat semen at refrigerated temperature. MATERIALS AND METHOD: For this experiment, semen was collected from eight Jakhrana bucks maintained at Jakhrana unit, ICAR-CIRG, at twice a week interval using artificial vagina. Collected semen was preliminary evaluated, and better semen samples were pooled and divided into two parts. One part of the pooled semen was diluted in egg yolk, Tris, citric acid, and fructose diluter, whereas second part was diluted in egg yolk, Tris, citric acid, and glucose diluter. Then semen samples were kept in equilibration chamber for 4 h at 5 °C after proper dilution. Both the semen samples were evaluated for viability, motility, plasma membrane integrity, sperm abnormality, lipid peroxidation, acrosomal integrity, and capacitation status at 0 h, 24 h, 48 h, and 72 h after dilution. RESULTS: Significantly (P < 0.05) higher motility was observed at 24 h in extender containing glucose as compared with extender containing fructose but motility was decreased at 48 h and 72 h. Number of capacitated sperm increased significantly (P < 0.05) and acrosomal integrity was decreased significantly (P < 0.05) at 72 h in extender containing glucose. The other parameters like viability and plasma membrane integrity were decreased significantly (P < 0.05) at 72 h and lipid peroxidation as well as sperm abnormality increased significantly (P < 0.05) in extender containing glucose. CONCLUSION: From this study, it can be concluded that fructose is better diluent sugar for refrigerated storage of buck semen.
OBJECTIVE: The aim of the study was to explore the possibility of a better sugar suitable for storage of goat semen at refrigerated temperature. MATERIALS AND METHOD: For this experiment, semen was collected from eight Jakhrana bucks maintained at Jakhrana unit, ICAR-CIRG, at twice a week interval using artificial vagina. Collected semen was preliminary evaluated, and better semen samples were pooled and divided into two parts. One part of the pooled semen was diluted in egg yolk, Tris, citric acid, and fructose diluter, whereas second part was diluted in egg yolk, Tris, citric acid, and glucose diluter. Then semen samples were kept in equilibration chamber for 4 h at 5 °C after proper dilution. Both the semen samples were evaluated for viability, motility, plasma membrane integrity, sperm abnormality, lipid peroxidation, acrosomal integrity, and capacitation status at 0 h, 24 h, 48 h, and 72 h after dilution. RESULTS: Significantly (P < 0.05) higher motility was observed at 24 h in extender containing glucose as compared with extender containing fructose but motility was decreased at 48 h and 72 h. Number of capacitated sperm increased significantly (P < 0.05) and acrosomal integrity was decreased significantly (P < 0.05) at 72 h in extender containing glucose. The other parameters like viability and plasma membrane integrity were decreased significantly (P < 0.05) at 72 h and lipid peroxidation as well as sperm abnormality increased significantly (P < 0.05) in extender containing glucose. CONCLUSION: From this study, it can be concluded that fructose is better diluent sugar for refrigerated storage of buck semen.
Authors: S Selvaraju; T Sivasubramani; B S Raghavendra; P Raju; S B N Rao; D Dineshkumar; J P Ravindra Journal: Theriogenology Date: 2012-05-22 Impact factor: 2.740
Authors: S W Naing; H Wahid; K Mohd Azam; Y Rosnina; A B Zuki; S Kazhal; M M Bukar; M Thein; T Kyaw; M M San Journal: Anim Reprod Sci Date: 2010-06-25 Impact factor: 2.145