Literature DB >> 3291639

A general procedure for screening inhibitory antibodies: application for identifying anti-protein kinase C antibodies.

D Mochly-Rosen1, D E Koshland.   

Abstract

In this communication we describe a microfiltration assay to identify monoclonal antibodies that interfere with the activity of enzymes. This method is quick and sensitive to small changes in the activity of the enzyme and does not require highly purified enzyme or large quantities of antibodies. It has been applied to identify anti-protein kinase C antibodies which would have been impossible to identify by classical assays such as enzyme-linked immunosorbent assay.

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Year:  1988        PMID： 3291639     DOI： 10.1016/0003-2697(88)90085-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  5 in total

1.  A protein kinase C isozyme is translocated to cytoskeletal elements on activation.

Authors:  D Mochly-Rosen; C J Henrich; L Cheever; H Khaner; P C Simpson
Journal:  Cell Regul       Date:  1990-08

2.  Identification of intracellular receptor proteins for activated protein kinase C.

Authors:  D Mochly-Rosen; H Khaner; J Lopez
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-01       Impact factor: 11.205

3.  Distribution of active protein kinase C in smooth muscle.

Authors:  G A Meininger; E D Moore; D J Schmidt; L M Lifshitz; F S Fay
Journal:  Biophys J       Date:  1999-08       Impact factor: 4.033

4.  Surface protein phosphorylation by ecto-protein kinase is required for the maintenance of hippocampal long-term potentiation.

Authors:  W Chen; A Wieraszko; M V Hogan; H A Yang; E Kornecki; Y H Ehrlich
Journal:  Proc Natl Acad Sci U S A       Date:  1996-08-06       Impact factor: 11.205

5.  Testing the in vivo role of protein kinase C and c-fos in neurite outgrowth by microinjection of antibodies into PC12 cells.

Authors:  J G Altin; R Wetts; K T Riabowol; R A Bradshaw
Journal:  Mol Biol Cell       Date:  1992-03       Impact factor: 4.138
  5 in total

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