Ryosuke Usui1, Tetsuya Ogawa2, Hideo Takahashi3, Chihiro Iwasaki4, Minako Koike4, Taku Morito5, Michiyasu Hatano5, Kosaku Nitta6. 1. Division of Nephrology, Department of Medicine, Yachiyo Medical Center, Tokyo Women's Medical University, 477-96 Owadashinden, Yachiyo city, Chiba, 276-8524, Japan. usui.ryosuke@twmu.ac.jp. 2. Department of Medicine, Tokyo Women's Medical University Medical Center East, Tokyo, Japan. 3. Minami-Akatsuka Clinic, Ibaraki, Japan. 4. Division of Nephrology, Department of Medicine, Yachiyo Medical Center, Tokyo Women's Medical University, 477-96 Owadashinden, Yachiyo city, Chiba, 276-8524, Japan. 5. Department of Medicine, Division of Nephrology, Yokohama Rosai Hospital, Kanagawa, Japan. 6. Department of Medicine, Kidney Center, Tokyo Women's Medical University, Tokyo, Japan.
Abstract
BACKGROUND: Uromodulin, also known as Tamm-Horsfall protein, is the most abundant protein in urine. It has recently been reported that uromodulin exists in a small amount in blood and that its concentration correlates with the estimated glomerular filtration rate (eGFR). METHODS: First, we generated anti-human uromodulin mouse monoclonal antibodies (mAb(s)) and established a specific enzyme-linked immunosorbent assay (ELISA) for uromodulin. We then performed an observational clinical study to determine if there was a correlation between serum uromodulin concentration and estimates of kidney function and whether the serum uromodulin value could be a biomarker in clinical nephrology. The clinical study included 308 patients with and without chronic kidney disease and healthy volunteers. Serum concentrations of creatinine, cystatin C, and uromodulin were measured and correlations were sought between the eGFR calculated from the creatinine and cystatin C levels and the serum uromodulin concentration. RESULTS: There was a good correlation between the serum uromodulin concentration and the eGFR value calculated from the creatinine (r = 0.76) and cystatin C (r = 0.79) levels. The mean serum uromodulin level in the group with an eGFR > 90 mL/min/1.73 m2 calculated using cystatin C was significantly higher than that in the group with an eGFR of 80-89 mL/min/1.73 m2. CONCLUSIONS: The serum uromodulin measurement could be a useful biomarker for identification of patients with early deterioration of kidney function.
BACKGROUND:Uromodulin, also known as Tamm-Horsfall protein, is the most abundant protein in urine. It has recently been reported that uromodulin exists in a small amount in blood and that its concentration correlates with the estimated glomerular filtration rate (eGFR). METHODS: First, we generated anti-human uromodulin mouse monoclonal antibodies (mAb(s)) and established a specific enzyme-linked immunosorbent assay (ELISA) for uromodulin. We then performed an observational clinical study to determine if there was a correlation between serum uromodulin concentration and estimates of kidney function and whether the serum uromodulin value could be a biomarker in clinical nephrology. The clinical study included 308 patients with and without chronic kidney disease and healthy volunteers. Serum concentrations of creatinine, cystatin C, and uromodulin were measured and correlations were sought between the eGFR calculated from the creatinine and cystatin C levels and the serum uromodulin concentration. RESULTS: There was a good correlation between the serum uromodulin concentration and the eGFR value calculated from the creatinine (r = 0.76) and cystatin C (r = 0.79) levels. The mean serum uromodulin level in the group with an eGFR > 90 mL/min/1.73 m2 calculated using cystatin C was significantly higher than that in the group with an eGFR of 80-89 mL/min/1.73 m2. CONCLUSIONS: The serum uromodulin measurement could be a useful biomarker for identification of patients with early deterioration of kidney function.
Authors: Lorenz Risch; Karl Lhotta; Dominik Meier; Pedro Medina-Escobar; Urs E Nydegger; Martin Risch Journal: Clin Chem Lab Med Date: 2014-12 Impact factor: 3.694
Authors: Danuta Fedak; Marek Kuźniewski; Andrzej Fugiel; Ewa Wieczorek-Surdacka; Bernadetta Przepiórkowska-Hoyer; Piotr Jasik; Przemysław Miarka; Paulina Dumnicka; Maria Kapusta; Bogdan Solnica; Władysław Sułowicz Journal: Pol Arch Med Wewn Date: 2016-12-05