New insights in the in vitro release of phenolic antioxidants: The case study of the release behavior of tyrosol from tyrosol-loaded ethylcellulose microparticles during the in vitro gastrointestinal digestion.

In this study, tyrosol - a phenolic antioxidant that present in olive oil and olive mill wastes - was embedded in ethylcellulose microparticles by double emulsion solvent evaporation technique. The effect of loading content (5 % w/w and 10 % w/w) on the release behavior and bioaccessibility of tyrosol was evaluated. The polymer endowed efficient protection to tyrosol during the in vitro gastrointestinal digestion of loaded microparticles as the maximum release of tyrosol was observed during the simulated intestinal digestion, and the releases were kept outstanding low during the simulated salivary and gastric digestions. The bioaccessibility of tyrosol was improved when encapsulated. The best-fitting models of the release profiles of tyrosol were the first, and the zero-order models for formulations considering a loading of 5% w/w and 10 % w/w, respectively. The results of this study bring new perspectives for the design of loaded microparticles that will be further submitted to gastrointestinal digestion.