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Literature DB >> 3290899

FLP recombinase is an enzyme.

Abstract

The FLP protein of the yeast 2-microns plasmid catalyzes intermolecular site-specific recombination with a turnover number of approximately equal to 0.12 min-1 (per FLP monomer) for relaxed DNA substrates. Under conditions that enhance its stability, the protein can be used in catalytic rather than stoichiometric amounts. The reaction rate exhibits a strong dependence on FLP protein concentration even when the protein is present in excess relative to available recombination sites.

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Year: 1988 PMID： 3290899 PMCID： PMC280488 DOI： 10.1073/pnas.85.13.4628

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

30 in total

1. Roles of the 2 microns gene products in stable maintenance of the 2 microns plasmid of Saccharomyces cerevisiae.

Authors: A E Reynolds; A W Murray; J W Szostak
Journal: Mol Cell Biol Date: 1987-10 Impact factor: 4.272

2. In vitro analysis of Hin-mediated site-specific recombination.

Authors: R C Johnson; M B Bruist; M B Glaccum; M I Simon
Journal: Cold Spring Harb Symp Quant Biol Date: 1984

3. Studies on the properties of P1 site-specific recombination: evidence for topologically unlinked products following recombination.

Authors: K Abremski; R Hoess; N Sternberg
Journal: Cell Date: 1983-04 Impact factor: 41.582

4. Purification and properties of the Escherichia coli protein factor required for lambda integrative recombination.

Authors: H A Nash; C A Robertson
Journal: J Biol Chem Date: 1981-09-10 Impact factor: 5.157

Review 5. Integration and excision of bacteriophage lambda: the mechanism of conservation site specific recombination.

Authors: H A Nash
Journal: Annu Rev Genet Date: 1981 Impact factor: 16.830

6. The FLP protein of the yeast 2-microns plasmid: expression of a eukaryotic genetic recombination system in Escherichia coli.

Authors: M M Cox
Journal: Proc Natl Acad Sci U S A Date: 1983-07 Impact factor: 11.205

7. Transposon Tn3 encodes a site-specific recombination system: identification of essential sequences, genes, and actual site of recombination.

Authors: R Kostriken; C Morita; F Heffron
Journal: Proc Natl Acad Sci U S A Date: 1981-07 Impact factor: 11.205

Review 9. The 2 micron plasmid: a selfish genetic element with an optimized survival strategy within Saccharomyces cerevisiae.

Authors: Syed Meraj Azhar Rizvi; Hemant Kumar Prajapati; Santanu Kumar Ghosh
Journal: Curr Genet Date: 2017-06-08 Impact factor: 3.886

10. Substrate features important for recognition and catalysis by human immunodeficiency virus type 1 integrase identified by using novel DNA substrates.

Authors: S A Chow; P O Brown
Journal: J Virol Date: 1994-06 Impact factor: 5.103

FLP recombinase is an enzyme.

1. Roles of the 2 microns gene products in stable maintenance of the 2 microns plasmid of Saccharomyces cerevisiae.

2. In vitro analysis of Hin-mediated site-specific recombination.

3. Studies on the properties of P1 site-specific recombination: evidence for topologically unlinked products following recombination.

4. Purification and properties of the Escherichia coli protein factor required for lambda integrative recombination.

Review 5. Integration and excision of bacteriophage lambda: the mechanism of conservation site specific recombination.

6. The FLP protein of the yeast 2-microns plasmid: expression of a eukaryotic genetic recombination system in Escherichia coli.

7. Transposon Tn3 encodes a site-specific recombination system: identification of essential sequences, genes, and actual site of recombination.

8. Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein.

9. Site-specific recombination of yeast 2-micron DNA in vitro.

10. Site-specific recombination by Gin of bacteriophage Mu: inversions and deletions.

1. Efficient gene activation in cultured mammalian cells mediated by FLP recombinase-expressing recombinant adenovirus.

2. A novel recombinator in yeast based on gene II protein from bacteriophage f1.

3. A bacterial model system for chromosomal targeting.

4. Characterization of Holliday structures in FLP protein-promoted site-specific recombination.

5. Different thermostabilities of FLP and Cre recombinases: implications for applied site-specific recombination.

6. Expression of Flp Protein in a Baculovirus/Insect Cell System for Biotechnological Applications.

7. Holliday intermediates and reaction by-products in FLP protein-promoted site-specific recombination.

8. SSV1-encoded site-specific recombination system in Sulfolobus shibatae.

Review 9. The 2 micron plasmid: a selfish genetic element with an optimized survival strategy within Saccharomyces cerevisiae.

10. Substrate features important for recognition and catalysis by human immunodeficiency virus type 1 integrase identified by using novel DNA substrates.