| Literature DB >> 32901605 |
Marcelo Rudzinski1,2,3, Lais Pardini3,4, Mariana Bernstein3,4, Gastón Moré3,4, Marina Khoury5, Silvana Carolina Duarte2, Carina Argüelles6, Silvia Reina2,3, José Raúl Oubiña7.
Abstract
Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.Entities:
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Year: 2020 PMID： 32901605 PMCID： PMC7695064 DOI： 10.4269/ajtmh.20-0124
Source DB: PubMed Journal: Am J Trop Med Hyg ISSN： 0002-9637 Impact factor: 3.707