The moso bamboo drought-induced 19 protein PheDi19-8 functions oppositely to its interacting partner, PheCDPK22, to modulate drought stress tolerance.

Drought-induced 19 (Di19) proteins play crucial roles in regulating stress responses, but the exact mechanisms underlying their involvement in moso bamboo are not fully understood. In this study, PheDi19-8 of moso bamboo (Phyllostachys edulis) was isolated and characterized. PheDi19-8 was a nuclear protein and has a high expression under various abiotic stresses, including drought and salt. As revealed by phenotypic and physiological analyses, ectopic overexpression of PheDi19-8 in Arabidopsis and rice enhanced drought tolerance. Under drought stress, the PheDi19-8-overexpressing lines showed smaller stomatal apertures and higher survival rate in comparison to the wild-type plants, as well as the PheDi19-8-overexpressing lines had higher biomass and souble sugar, but lower relative electrolyte leakage and malondialdehyde. Further investigation revealed that PheDi19-8 interacted with PheCDPK22, and their interaction decreased the DNA-binding activity of PheDi19-8. However, overexpression of PheCDPK22 enhanced Arabidopsis sensitivity to drought stress. Moreover, the expression of marker genes, including LEA, RD22, DREB2A and RD29A, was up-regulated in the PheDi19-8-overexpressing lines but down-regulated in the PheCDPK22-overexpressing. Further yeast one-hybrid and EMSA assays indicated that PheDi19-8 directly binds to the promoter of DREB2A. These results provided new insight into the interaction of PheCDPK22 and PheDi19-8 that functions oppositely to regulate drought stress in plants.