Immunochemical analysis of extracellular matrix during embryonic lens development of the Cat Fraser mouse.

We have characterized the extracellular matrix present during early mouse-lens morphogenesis in Swiss and Cat Fraser mutant mice which produces a thicker capsule. In the two mouse strains, laminin was first detected when the optic vesicle and the head ectoderm are closely associated. At day 10, staining for laminin and fibronectin is especially concentrated at the border of the lens pit. At this stage, type IV collagen and proteoheparan sulphate have a similar distribution to laminin and fibronectin. In the two mouse strains, no major differences were observed in the intensity and the distribution of fluorescent basement-membrane components. This suggests that the overall increase in capsule thickness of the Cat Fraser mutant is more related to an increased cellular synthesis of capsule than to an abnormal distribution of one or more basement-membrane macromolecules.