Asmaa Kamal1, Mohamed Salama2, Amr Kamal2, Ahmed Mohsen3, Ibrahem Siam4. 1. Department of Clinical - Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt. 2. Department of Surgical Oncology, National Cancer Institute, Cairo Univeristy, Cairo, Egypt. 3. General Surgery Researcher, National Research Centre, Cairo, Egypt. 4. Department of Internal Medicine, National Research Centre, Cairo, Egypt.
Abstract
BACKGROUND/AIMS: Whereas colorectal cancer (CRC) is the third most common cancer worldwide, klotho gene has been reported as a tumor suppressor gene. Therefore, the aim of this study was to investigate the association between klotho (rs1207568 and rs564481) variants and CRC in Egyptian patients. MATERIALS AND METHODS: A case-control study comprising 100 patients with CRC and 100 age- and sex-matched healthy controls was conducted. Genotyping of klotho was performed by polymerase chain reaction with confronting two-pair primers. RESULTS: The frequencies of the A allele of rs1207568 and the AC haplotype were significantly higher in patients with CRC than in the controls (p=0.019 and p=0.005, respectively). CONCLUSION: We propose that klotho (rs1207568 and rs564481) variants play a significant role in colorectal carcinogenesis and that the klotho protein could be a target for oncotherapy.
BACKGROUND/AIMS: Whereas colorectal cancer (CRC) is the third most common cancer worldwide, klotho gene has been reported as a tumor suppressor gene. Therefore, the aim of this study was to investigate the association between klotho (rs1207568 and rs564481) variants and CRC in Egyptian patients. MATERIALS AND METHODS: A case-control study comprising 100 patients with CRC and 100 age- and sex-matched healthy controls was conducted. Genotyping of klotho was performed by polymerase chain reaction with confronting two-pair primers. RESULTS: The frequencies of the A allele of rs1207568 and the AC haplotype were significantly higher in patients with CRC than in the controls (p=0.019 and p=0.005, respectively). CONCLUSION: We propose that klotho (rs1207568 and rs564481) variants play a significant role in colorectal carcinogenesis and that the klotho protein could be a target for oncotherapy.
Authors: I Wolf; S Levanon-Cohen; S Bose; H Ligumsky; B Sredni; H Kanety; M Kuro-o; B Karlan; B Kaufman; H P Koeffler; T Rubinek Journal: Oncogene Date: 2008-09-01 Impact factor: 9.867