Literature DB >> 3289547

[Cloning, expression and structure of the functionally active shortened lon gene in Escherichia coli].

A Iu Amerik, L G Chistiakov, N I Ostroumova, A I Gurevich, V K Antonov.   

Abstract

Lon gene of E. coli has been cloned into the plasmid pBR327. Full nucleotide sequence of the gene has been established. It was shown that the cloned gene does not possess the terminal codon and is somewhat shortened. Nevertheless it retains full phenotypic activity and expresses the C-end modified La proteinase which retains ATP-dependent proteolytic activity.

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Year:  1988        PMID： 3289547

Source DB:  PubMed          Journal:  Bioorg Khim        ISSN： 0132-3423


  10 in total

Review 1.  Slicing a protease: structural features of the ATP-dependent Lon proteases gleaned from investigations of isolated domains.

Authors:  Tatyana V Rotanova; Istvan Botos; Edward E Melnikov; Fatima Rasulova; Alla Gustchina; Michael R Maurizi; Alexander Wlodawer
Journal:  Protein Sci       Date:  2006-08       Impact factor: 6.725

2.  Single-turnover kinetic experiments confirm the existence of high- and low-affinity ATPase sites in Escherichia coli Lon protease.

Authors:  Diana Vineyard; Jessica Patterson-Ward; Irene Lee
Journal:  Biochemistry       Date:  2006-04-11       Impact factor: 3.162

3.  N-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases.

Authors:  A E Gorbalenya; A P Donchenko; E V Koonin; V M Blinov
Journal:  Nucleic Acids Res       Date:  1989-05-25       Impact factor: 16.971

4.  Cloning, nucleotide sequence, and expression of the Bacillus subtilis lon gene.

Authors:  S Riethdorf; U Völker; U Gerth; A Winkler; S Engelmann; M Hecker
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

5.  Utilization of synthetic peptides to evaluate the importance of substrate interaction at the proteolytic site of Escherichia coli Lon protease.

Authors:  Jessica Patterson-Ward; Johnathan Tedesco; Jason Hudak; Jennifer Fishovitz; James Becker; Hilary Frase; Kirsten McNamara; Irene Lee
Journal:  Biochim Biophys Acta       Date:  2009-03-11

6.  Cloning and nucleotide sequence of the Myxococcus xanthus lon gene: indispensability of lon for vegetative growth.

Authors:  N Tojo; S Inouye; T Komano
Journal:  J Bacteriol       Date:  1993-04       Impact factor: 3.490

7.  The lonD gene is homologous to the lon gene encoding an ATP-dependent protease and is essential for the development of Myxococcus xanthus.

Authors:  N Tojo; S Inouye; T Komano
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

8.  Myxococcus xanthus encodes an ATP-dependent protease which is required for developmental gene transcription and intercellular signaling.

Authors:  R E Gill; M Karlok; D Benton
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

9.  Detection and characterization of two ATP-dependent conformational changes in proteolytically inactive Escherichia coli Lon mutants by stopped flow kinetic techniques.

Authors:  Jessica Patterson-Ward; Jon Huang; Irene Lee
Journal:  Biochemistry       Date:  2007-11-02       Impact factor: 3.162

Review 10.  Molecular and biotechnological aspects of microbial proteases.

Authors:  M B Rao; A M Tanksale; M S Ghatge; V V Deshpande
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056
  10 in total

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