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Literature DB >> 32895122

[Application of laboratory diagnostic technologies for SARS-CoV-2: current progress and prospect].

Bin Xiao¹, Quan Zhou¹, Ting Lei¹, Yongyin He¹, Linhai Li¹.

Abstract

Since the outbreak of COVID-19 pandemic, the detection capability has been improving and the detection techniques have been evolving with innovations. qRT- PCR and mNGS, which represent the current mainstay diagnostic technologies, play key roles in disease diagnosis and monitoring of virus variation. The detection technologies based on serum and plasma IgM and IgG antibodies are important for auxiliary diagnosis. RT-LAMP is highly specific for a diagnostic purpose. Digital PCR could quantitatively detect nucleic acid and SHERLOCK has a higher sensitivity. These techniques all have great potential for future development and application for pathogen detection. In this review the authors summarize the basic rationales, technical characteristics and the current application of the SARS-CoV-2 detection techniques.

Entities: Disease Species

Keywords: SARS-Cov-2; SHERLOCK; antibody; digital PCR; qRT-PCR

Mesh：

Substances：

Year: 2020 PMID： 32895122 PMCID： PMC7225119 DOI： 10.12122/j.issn.1673-4254.2020.04.24

Source DB: PubMed Journal: Nan Fang Yi Ke Da Xue Xue Bao ISSN： 1673-4254

11 in total

1. Nucleic acid detection with CRISPR-Cas13a/C2c2.

Authors: Jonathan S Gootenberg; Omar O Abudayyeh; Jeong Wook Lee; Patrick Essletzbichler; Aaron J Dy; Julia Joung; Vanessa Verdine; Nina Donghia; Nichole M Daringer; Catherine A Freije; Cameron Myhrvold; Roby P Bhattacharyya; Jonathan Livny; Aviv Regev; Eugene V Koonin; Deborah T Hung; Pardis C Sabeti; James J Collins; Feng Zhang
Journal: Science Date: 2017-04-13 Impact factor: 47.728

2. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.

Authors: Sudhir Kumar; Glen Stecher; Michael Li; Christina Knyaz; Koichiro Tamura
Journal: Mol Biol Evol Date: 2018-06-01 Impact factor: 16.240

3. Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia.

Authors: Daniel K W Chu; Yang Pan; Samuel M S Cheng; Kenrie P Y Hui; Pavithra Krishnan; Yingzhi Liu; Daisy Y M Ng; Carrie K C Wan; Peng Yang; Quanyi Wang; Malik Peiris; Leo L M Poon
Journal: Clin Chem Date: 2020-04-01 Impact factor: 8.327

4. Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6.

Authors: Jonathan S Gootenberg; Omar O Abudayyeh; Max J Kellner; Julia Joung; James J Collins; Feng Zhang
Journal: Science Date: 2018-02-15 Impact factor: 47.728

5. Field-deployable viral diagnostics using CRISPR-Cas13.

Authors: Cameron Myhrvold; Catherine A Freije; Jonathan S Gootenberg; Omar O Abudayyeh; Hayden C Metsky; Ann F Durbin; Max J Kellner; Amanda L Tan; Lauren M Paul; Leda A Parham; Kimberly F Garcia; Kayla G Barnes; Bridget Chak; Adriano Mondini; Mauricio L Nogueira; Sharon Isern; Scott F Michael; Ivette Lorenzana; Nathan L Yozwiak; Bronwyn L MacInnis; Irene Bosch; Lee Gehrke; Feng Zhang; Pardis C Sabeti
Journal: Science Date: 2018-04-27 Impact factor: 47.728

6. RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak.

Authors: Liangjun Chen; Weiyong Liu; Qi Zhang; Ke Xu; Guangming Ye; Weichen Wu; Ziyong Sun; Fang Liu; Kailang Wu; Bo Zhong; Yi Mei; Wenxia Zhang; Yu Chen; Yirong Li; Mang Shi; Ke Lan; Yingle Liu
Journal: Emerg Microbes Infect Date: 2020-02-05 Impact factor: 7.163

7. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding.

Authors: Roujian Lu; Xiang Zhao; Juan Li; Peihua Niu; Bo Yang; Honglong Wu; Wenling Wang; Hao Song; Baoying Huang; Na Zhu; Yuhai Bi; Xuejun Ma; Faxian Zhan; Liang Wang; Tao Hu; Hong Zhou; Zhenhong Hu; Weimin Zhou; Li Zhao; Jing Chen; Yao Meng; Ji Wang; Yang Lin; Jianying Yuan; Zhihao Xie; Jinmin Ma; William J Liu; Dayan Wang; Wenbo Xu; Edward C Holmes; George F Gao; Guizhen Wu; Weijun Chen; Weifeng Shi; Wenjie Tan
Journal: Lancet Date: 2020-01-30 Impact factor: 79.321

8. Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis.

Authors: Zhengtu Li; Yongxiang Yi; Xiaomei Luo; Nian Xiong; Yang Liu; Shaoqiang Li; Ruilin Sun; Yanqun Wang; Bicheng Hu; Wei Chen; Yongchen Zhang; Jing Wang; Baofu Huang; Ye Lin; Jiasheng Yang; Wensheng Cai; Xuefeng Wang; Jing Cheng; Zhiqiang Chen; Kangjun Sun; Weimin Pan; Zhifei Zhan; Liyan Chen; Feng Ye
Journal: J Med Virol Date: 2020-04-13 Impact factor: 2.327

9. Molecular characterization of SARS-CoV-2 in the first COVID-19 cluster in France reveals an amino acid deletion in nsp2 (Asp268del).

Authors: A Bal; G Destras; A Gaymard; M Bouscambert-Duchamp; M Valette; V Escuret; E Frobert; G Billaud; S Trouillet-Assant; V Cheynet; K Brengel-Pesce; F Morfin; B Lina; L Josset
Journal: Clin Microbiol Infect Date: 2020-03-28 Impact factor: 8.067