Literature DB >> 32894756

Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2.

Jim F Huggett1,2, Vladimir Benes3, Stephen A Bustin4, Jeremy A Garson5, Karthyn Harris6, Martin Kammel7, Mikael Kubista8, Timothy D McHugh9, Jacob Moran-Gilad10, Tania Nolan11, Michael W Pfaffl12, Marc Salit13,14, Greg Shipley15, Peter M Vallone16, Jo Vandesompele17,18, Carl Wittwer19, Heinz Zeichhardt20.   

Abstract

Entities:  

Keywords:  RT-qPCR; SARS-CoV-2; contamination; false positive; molecular diagnosis

Mesh：

Substances：

Year:  2020        PMID： 32894756      PMCID： PMC7499524          DOI： 10.1093/clinchem/hvaa214

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


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  11 in total

1.  Prevalence of SARS-CoV-2 Infection Among COVID-19 Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Laboratory Workers in Bangladesh.

Authors:  Mohammad Jahidur Rahman Khan; Samshad Jahan Shumu; Farzana Mim; Ruksana Raihan; Nusrat Mannan; Md Selim Reza; Nazia Hasan Khan; Arifa Akram; Amirul Huda Bhuiyan; Paroma Deb
Journal:  Cureus       Date:  2022-04-17

2.  False-Positive Results of SARS-CoV-2 RT-PCR in Oropharyngeal Swabs From Vaccinators.

Authors:  Xiang-Qi Kong; Yong-Jing Wang; Zan-Xi Fang; Tian-Ci Yang; Man-Li Tong
Journal:  Front Med (Lausanne)       Date:  2022-06-10

3.  Digital PCR can augment the interpretation of RT-qPCR Cq values for SARS-CoV-2 diagnostics.

Authors:  Alexandra S Whale; Eva K von der Heide; Max Kohlenberg; Anja Brinckmann; Silke Baedker; Oezlem Karalay; Ana Fernandez-Gonzalez; Eloise J Busby; Stephen A Bustin; Heiko Hauser; Andreas Missel; Denise M O'Sullivan; Jim F Huggett; Michael W Pfaffl; Tania Nolan
Journal:  Methods       Date:  2021-08-26       Impact factor: 4.647

Review 4.  Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements.

Authors:  Mojca Milavec; Megan H Cleveland; Young-Kyung Bae; Robert I Wielgosz; Maxim Vonsky; Jim F Huggett
Journal:  Anal Bioanal Chem       Date:  2021-11-04       Impact factor: 4.142

5.  Update of Guidelines for Laboratory Diagnosis of COVID-19 in Korea.

Authors:  Ki Ho Hong; Gab Jung Kim; Kyoung Ho Roh; Heungsup Sung; Jaehyeon Lee; So Yeon Kim; Taek Soo Kim; Jae-Sun Park; Hee Jae Huh; Younhee Park; Jae-Seok Kim; Hyun Soo Kim; Moon-Woo Seong; Nam Hee Ryoo; Sang Hoon Song; Hyukmin Lee; Gye Cheol Kwon; Cheon Kwon Yoo
Journal:  Ann Lab Med       Date:  2022-07-01       Impact factor: 4.941

6.  Development of a high sensitivity RT-PCR assay for detection of SARS-CoV-2 in individual and pooled nasopharyngeal samples.

Authors:  Harindi Jayakody; Daniel Rowland; Clint Pereira; Rachel Blackwell; Tomasz Lasota; Mark Laverick; Laurence Tisi; Hannah S Leese; Alistair D S Walsham
Journal:  Sci Rep       Date:  2022-03-30       Impact factor: 4.379

7.  Sequential development of several RT-qPCR tests using LNA nucleotides and dual probe technology to differentiate SARS-CoV-2 from influenza A and B.

Authors:  Monika Radvánszka; Evan D Paul; Roman Hajdu; Kristína Boršová; Viera Kováčová; Piotr Putaj; Stanislava Bírová; Ivana Čirková; Martin Čarnecký; Katarína Buranovská; Adrián Szobi; Nina Vojtaššáková; Diana Drobná; Viktória Čabanová; Monika Sláviková; Martina Ličková; Veronika Vaňová; Sabína Fumačová Havlíková; Ľubomíra Lukáčiková; Ivana Kajanová; Juraj Koči; Diana Rusňáková; Tatiana Sedláčková; Klaas E A Max; Thomas Tuschl; Tomáš Szemes; Boris Klempa; Pavol Čekan
Journal:  Microb Biotechnol       Date:  2022-03-22       Impact factor: 6.575

8.  Monkeypox: another test for PCR.

Authors:  Jim F Huggett; David French; Denise M O'Sullivan; Jacob Moran-Gilad; Alimuddin Zumla
Journal:  Euro Surveill       Date:  2022-08

9.  Guidelines on newly identified limitations of diagnostic tools for COVID-19 and consequences.

Authors:  Kirtimaan Syal
Journal:  J Med Virol       Date:  2020-12-17       Impact factor: 20.693

10.  Development of robust isothermal RNA amplification assay for lab-free testing of RNA viruses.

Authors:  Radhika Biyani; Kirti Sharma; Kenji Kojima; Madhu Biyani; Vishnu Sharma; Tarun Kumawat; Kevin Maafu Juma; Itaru Yanagihara; Shinsuke Fujiwara; Eiichi Kodama; Yuzuru Takamura; Masahiro Takagi; Kiyoshi Yasukawa; Manish Biyani
Journal:  Sci Rep       Date:  2021-08-06       Impact factor: 4.379
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