Literature DB >> 3289119

Production of stable rabbit-mouse hybridomas that secrete rabbit mAb of defined specificity.

T J Raybould1, M Takahashi.   

Abstract

Inclusion of normal rabbit serum (NRS) in culture medium after interspecific fusion of hyperimmunized rabbit spleen cells with murine SP2/0 myeloma cells produced 271 rabbit-mouse hybridomas (RMHs) that secreted rabbit immunoglobulin against group A Streptococcus (GAS). Continued use of NRS-supplemented medium during cloning yielded stabilized monoclonal RMH lines that have secreted GAS-specific rabbit antibody at concentrations similar to murine hybridomas (3 to 8 micrograms per 10(6) cells per 24 hours), for over 4 months of culture in vitro. The use of NRS as a medium supplement during initial culture, cloning, and stabilization of RMHs enables production of considerably more specific rabbit monoclonal antibody (mAb)-secreting RMHs than have previously been reported.

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Year:  1988        PMID： 3289119     DOI： 10.1126/science.3289119

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  11 in total

1.  Rabbit monoclonal antibody: potential application in cancer therapy.

Authors:  Lifeng Feng; Xian Wang; Hongchuan Jin
Journal:  Am J Transl Res       Date:  2011-04-23       Impact factor: 4.060

Review 2.  The production and application of non-rodent monoclonal antibodies in veterinary science.

Authors:  D J Groves; E M Tucker
Journal:  Vet Immunol Immunopathol       Date:  1989-11-30       Impact factor: 2.046

3.  Rabbit monoclonal antibodies: generating a fusion partner to produce rabbit-rabbit hybridomas.

Authors:  H Spieker-Polet; P Sethupathi; P C Yam; K L Knight
Journal:  Proc Natl Acad Sci U S A       Date:  1995-09-26       Impact factor: 11.205

Review 4.  Advances in the Isolation of Specific Monoclonal Rabbit Antibodies.

Authors:  Zaibao Zhang; Huijuan Liu; Qian Guan; Lei Wang; Hongyu Yuan
Journal:  Front Immunol       Date:  2017-05-05       Impact factor: 7.561

5.  Monoclonal Suncus Antibodies: Generation of Fusion Partners to Produce Suncus-Suncus Hybridomas.

Authors:  Yoshikazu Sado; Satoko Inoue; Yasuko Tomono; Makoto Matsuyama; Masaki Fukushima; Toshitaka Oohashi; Takamichi Jogahara; Sen-Ichi Oda
Journal:  Acta Histochem Cytochem       Date:  2017-04-15       Impact factor: 1.938

Review 6.  From rabbit antibody repertoires to rabbit monoclonal antibodies.

Authors:  Justus Weber; Haiyong Peng; Christoph Rader
Journal:  Exp Mol Med       Date:  2017-03-24       Impact factor: 8.718

Review 7.  Hybridoma technology a versatile method for isolation of monoclonal antibodies, its applicability across species, limitations, advancement and future perspectives.

Authors:  Hilal Ahmed Parray; Shivangi Shukla; Sweety Samal; Tripti Shrivastava; Shubbir Ahmed; Chandresh Sharma; Rajesh Kumar
Journal:  Int Immunopharmacol       Date:  2020-05-27       Impact factor: 4.932

8.  A novel rabbit immunospot array assay on a chip allows for the rapid generation of rabbit monoclonal antibodies with high affinity.

Authors:  Tatsuhiko Ozawa; Xiuhong Piao; Eiji Kobayashi; Yue Zhou; Hiroaki Sakurai; Tsugunobu Andoh; Aishun Jin; Hiroyuki Kishi; Atsushi Muraguchi
Journal:  PLoS One       Date:  2012-12-26       Impact factor: 3.240

9.  Generation of Recombinant Monoclonal Antibodies from Immunised Mice and Rabbits via Flow Cytometry and Sorting of Antigen-Specific IgG+ Memory B Cells.

Authors:  Dale O Starkie; Joanne E Compson; Stephen Rapecki; Daniel J Lightwood
Journal:  PLoS One       Date:  2016-03-29       Impact factor: 3.240

10.  Optimization of culture conditions for feline x murine heterohybridomas.

Authors:  E Leidinger; E Kramberger-Kaplan; M Gemeiner
Journal:  Comp Immunol Microbiol Infect Dis       Date:  1993-10       Impact factor: 2.268
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