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Literature DB >> 3288992

Analysis in serum-free culture of the targets of recombinant human hemopoietic growth factors: interleukin 3 and granulocyte/macrophage-colony-stimulating factor are specific for early developmental stages.

Y Sonoda¹, Y C Yang, G G Wong, S C Clark, M Ogawa.

Abstract

We have used a serum-free culture system for enriched human hemopoietic progenitors to analyze the developmental stages and lineage specificities of the human hemopoietic colony-stimulating factors. None of the individual factors alone efficiently supported hemopoietic colony formation. Neither interleukin 3 nor granulocyte/macrophage-colony-stimulating factor alone or in combination effectively supported proliferation of progenitor cells. However, when combined with granulocyte-colony-stimulating factor or erythropoietin, these factors yielded neutrophil colonies or erythroid bursts, respectively. Serial observations of interleukin 3-supported cultures revealed sequential emergence and subsequent degeneration of clusters of cells. These observations suggest that the primary targets of interleukin 3 and granulocyte/macrophage-colony-stimulating factor are multipotent progenitors at the early stages of development rather than cells in the terminal process of maturation.

Entities: Gene Species

Mesh：

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Year: 1988 PMID： 3288992 PMCID： PMC280428 DOI： 10.1073/pnas.85.12.4360

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

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Journal: Blood Date: 1985-12 Impact factor: 22.113

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Journal: Blood Date: 1984-07 Impact factor: 22.113

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Journal: J Immunol Date: 1983-07 Impact factor: 5.422

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Authors: D Metcalf; C G Begley; G R Johnson; N A Nicola; M A Vadas; A F Lopez; D J Williamson; G G Wong; S C Clark; E A Wang
Journal: Blood Date: 1986-01 Impact factor: 22.113

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Journal: J Immunol Date: 1985-02 Impact factor: 5.422

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Authors: T Suda; J Suda; M Ogawa; J N Ihle
Journal: J Cell Physiol Date: 1985-08 Impact factor: 6.384

26 in total

1. Clonogenic assay of leukemic cells in serum-free culture.

Authors: Y C Dai; X Q Lu; Z Fang
Journal: In Vitro Cell Dev Biol Date: 1992 Nov-Dec

2. Biological inducers of haemopoietic differentiation.

Authors: S Eridani; E Abood
Journal: Cytotechnology Date: 1991-02 Impact factor: 2.058

3. Combination of interleukins 3 and 6 preserves stem cell function in culture and enhances retrovirus-mediated gene transfer into hematopoietic stem cells.

Authors: D M Bodine; S Karlsson; A W Nienhuis
Journal: Proc Natl Acad Sci U S A Date: 1989-11 Impact factor: 11.205

4. Comparative analysis of the influences of IL-1, IL-3 and GM-CSF on the commitment of granulocyte-macrophage progenitors in vitro.

Authors: J Maciejewski; H Weber; K Neuhaus; M Wächter; E Elstner; F R Seiler; H D Volk
Journal: Ann Hematol Date: 1991-11 Impact factor: 3.673

5. Effects of granulocyte/macrophage colony-stimulating factor on the development and differentiation of CD5-positive macrophages and their potential derivation from a CD5-positive B-cell lineage in mice.

Authors: K Takahashi; K Miyakawa; A A Wynn; K Nakayama; Y Y Myint; M Naito; L D Shultz; A Tominaga; K Takatsu
Journal: Am J Pathol Date: 1998-02 Impact factor: 4.307