José L Soriano1, Ana C Calpena2,3, María Rincón2, Noelia Pérez1, Lyda Halbaut2,3, María J Rodríguez-Lagunas4,5, Beatriz Clares1,3,6. 1. Department of Pharmacy & Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, 18071, Granada, Spain. 2. Department of Pharmacy & Pharmaceutical Technology & Physical Chemistry, Faculty of Pharmacy & Food Sciences, University of Barcelona, 08028, Barcelona, Spain. 3. Nanoscience & Nanotechnology Institute (IN2UB), University of Barcelona, 08028, Barcelona, Spain. 4. Department of Biochemistry & Physiology, Faculty of Pharmacy & Food Sciences, University of Barcelona, 08028, Barcelona, Spain. 5. Institute of Biomedicine, University of Barcelona, 08028, Barcelona, Spain. 6. Biosanitary Institute of Granada (ibs.GRANADA), 18012, Granada, Spain.
Abstract
Aim: Development of a melatonin nanogel intended for wound healing (WH) application. Materials & methods: The main components of the nanogel were poloxamer 407, chitosan and hyaluronic acid. The nanogel was characterized by the assessment of physical interactions, swelling, wettability, rheological properties and internal structure. The drug release, antimicrobial efficacy against different strains and biocompatibility with human keratinocytes were also tested. Finally, the WH efficacy was evaluated in rats. Results: The nanogel showed optimal physicochemical properties and an internal network of interconnected channels from which melatonin was released following first order kinetics. Antimicrobial activity was similar to commercial reference material and it promoted epidermis growth with evident wound contraction. Conclusion: Melatonin nanogel can be proposed as a promising system for WH.
Aim: Development of a melatonin nanogel intended for wound healing (WH) application. Materials & methods: The main components of the nanogel were poloxamer 407, chitosan and hyaluronic acid. The nanogel was characterized by the assessment of physical interactions, swelling, wettability, rheological properties and internal structure. The drug release, antimicrobial efficacy against different strains and biocompatibility with human keratinocytes were also tested. Finally, the WH efficacy was evaluated in rats. Results: The nanogel showed optimal physicochemical properties and an internal network of interconnected channels from which melatonin was released following first order kinetics. Antimicrobial activity was similar to commercial reference material and it promoted epidermis growth with evident wound contraction. Conclusion:Melatonin nanogel can be proposed as a promising system for WH.