Peng Song1, Chen Sun2, Jinbo Li3, Tong Long4, Yixin Yan5, Hongping Qin5, Emmanuel Ayobami Makinde6, Ademola C Famurewa7, Amit Jaisi8, Ying Nie9, Opeyemi Joshua Olatunji6. 1. Department of Urology, First Affiliated Hospital of Hainan Medical College, Haikou City, China. 2. Department of Internal Medicine, Yantai Municipal Government Hospital, Yantai, China. 3. Department of Endocrinology, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Jilin, China. 4. Department of Otolaryngology, Haikou Hospital of Traditional Chinese Medicine, Haikou City, China. 5. Department of Reproductive Centre, The 940th Hospital of Joint Logistics Support Force of Chinese PLA, Lanzhou, China. 6. Faculty of Thai Traditional Medicine, Prince of Songkla University, Hat Yai, Thailand. 7. Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Alex Ekwueme Federal University, Ikwo, Nigeria. 8. Department of Industrial Pharmacy, School of Pharmacy, Walailak University, Thasala, Nakhon Si Thammarat, Thailand. 9. Navy Outpatient Department Medicine Clinic of Fengtai Health Centre, Beijing, China.
Abstract
BACKGROUND: Literature has demonstrated that diabetes is associated with renal complication and testicular dysfunctions. The current study explored the potential of Tiliacora triandra extract and its major component against diabetic kidney and testicular damages in rats. METHODS: Diabetes was induced by high fat diet/streptozotocin (HFD/STZ) and treated orally with Tiliacora triandra extract (TTE, 100 and 400 mg kg-1 body weight) and its major component, 5,7-dihydroxy-6-oxoheptadecanoic acid (DHA, 25 mg kg-1 body weight) for 30 consecutive days. Testicular activities of testicular enzymes, serum levels of testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), sperm parameters and urinalysis for protein and albumin levels were evaluated. Renal and testicular biomarkers of oxidative stress and pro-inflammation were analysed along with histology. RESULTS: The experimental diabetes induced significant alterations in the levels and activities of indices evaluated compared to non-diabetic normal rats. The 28-day treatment of diabetic rats with TTE and DHA markedly improved activities of testicular enzymes, restored levels of testosterone, LH and FSH and sperm parameters compared to untreated diabetic rats. TTE and DHA abrogated proteinuria and reversed urine albumin level. Testicular and renal oxidative stress and pro-inflammation were attenuated in diabetic rats treated with TTE and DHA. The diabetes-mediated histopathological damage was alleviated in the kidney and testis. CONCLUSION: The protective effect of TTE and DHA against diabetes induced kidney and testicular damages may be related to its antioxidant and anti-inflammatory activities.
BACKGROUND: Literature has demonstrated that diabetes is associated with renal complication and testicular dysfunctions. The current study explored the potential of Tiliacora triandra extract and its major component against diabetic kidney and testicular damages in rats. METHODS:Diabetes was induced by high fat diet/streptozotocin (HFD/STZ) and treated orally with Tiliacora triandra extract (TTE, 100 and 400 mg kg-1 body weight) and its major component, 5,7-dihydroxy-6-oxoheptadecanoic acid (DHA, 25 mg kg-1 body weight) for 30 consecutive days. Testicular activities of testicular enzymes, serum levels of testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), sperm parameters and urinalysis for protein and albumin levels were evaluated. Renal and testicular biomarkers of oxidative stress and pro-inflammation were analysed along with histology. RESULTS: The experimental diabetes induced significant alterations in the levels and activities of indices evaluated compared to non-diabetic normal rats. The 28-day treatment of diabeticrats with TTE and DHA markedly improved activities of testicular enzymes, restored levels of testosterone, LH and FSH and sperm parameters compared to untreated diabeticrats. TTE and DHA abrogated proteinuria and reversed urine albumin level. Testicular and renal oxidative stress and pro-inflammation were attenuated in diabeticrats treated with TTE and DHA. The diabetes-mediated histopathological damage was alleviated in the kidney and testis. CONCLUSION: The protective effect of TTE and DHA against diabetes induced kidney and testicular damages may be related to its antioxidant and anti-inflammatory activities.
Authors: Isaac Kyei-Barffour; Roselind Kyei Baah Kwarkoh; Ophelia Duke Arthur; Samuel Addo Akwetey; Desmond Omane Acheampong; Benjamin Aboagye; Augustine Suurinobah Brah; Isaac Kingsley Amponsah; Christian Kweku Adokoh Journal: Heliyon Date: 2021-06-30