Sei Ueda1,2, Kengo Hashimoto1,2, Satoru Miyabe1, Shogo Hasegawa1, Mitsuo Goto1, Dai Shimizu2,3, Ichiro Oh-Iwa4, Kazuo Shimozato1, Toru Nagao1, Shuji Nomoto5. 1. Department of Maxillofacial Surgery, School of Dentistry, Aichi-gakuin University Graduate School of Medicine, Nagoya, Japan. 2. Department of Surgery, School of Dentistry, Aichi-gakuin University Graduate School of Medicine, Nagoya, Japan. 3. Department of Gastroenterological Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan. 4. Department of Maxillofacial Surgery, Japanese Red Cross Nagoya Daiichi Hospital, Nagoya, Japan. 5. Department of Surgery, School of Dentistry, Aichi-gakuin University Graduate School of Medicine, Nagoya, Japan snomoto@dpc.agu.ac.jp.
Abstract
BACKGROUND/AIM: Oral cancer may become advanced because of delay in diagnosis. In order to promote oral cancer screening, simple and highly reliable screening methods that can be implemented at general dental clinics are required. Herein we investigated differential salivary gene expression between oral squamous cell carcinoma (OSCC) patients and healthy volunteers (HV) to identify new biomarkers for OSCC detection. MATERIALS AND METHODS: Candidate genes were selected by microarrays, nuclear undecaprenyl pyrophosphate synthase 1 (NUS1) and reticulocalbin 1 (RCN1) were selected for further investigation. We used real-time quantitative reverse transcription PCR (qRT-PCR) to determine NUS1 and RCN1 expression levels in saliva and tissues. RESULTS: qRT-PCR analysis of clinical samples revealed that OSCC patients had significantly higher expression of salivary NUS1 and RCN1 than HV. CONCLUSION: A combination of NUS1 and RCN1 accurately distinguished patients from controls, and this combination can be implemented as a screening test for OSCC. Copyright
BACKGROUND/AIM: Oral cancer may become advanced because of delay in diagnosis. In order to promote oral cancer screening, simple and highly reliable screening methods that can be implemented at general dental clinics are required. Herein we investigated differential salivary gene expression between oral squamous cell carcinoma (OSCC) patients and healthy volunteers (HV) to identify new biomarkers for OSCC detection. MATERIALS AND METHODS: Candidate genes were selected by microarrays, nuclear undecaprenyl pyrophosphate synthase 1 (NUS1) and reticulocalbin 1 (RCN1) were selected for further investigation. We used real-time quantitative reverse transcription PCR (qRT-PCR) to determine NUS1 and RCN1 expression levels in saliva and tissues. RESULTS: qRT-PCR analysis of clinical samples revealed that OSCC patients had significantly higher expression of salivary NUS1 and RCN1 than HV. CONCLUSION: A combination of NUS1 and RCN1 accurately distinguished patients from controls, and this combination can be implemented as a screening test for OSCC. Copyright
Authors: Allen S W Oak; Georgeta Bocheva; Tae-Kang Kim; Anna A Brożyna; Zorica Janjetovic; Mohammad Athar; Robert C Tuckey; Andrzej T Slominski Journal: Anticancer Res Date: 2020-05 Impact factor: 2.480
Authors: B Zhao; W Hu; S Kumar; P Gonyo; U Rana; Z Liu; B Wang; W Q Duong; Z Yang; C L Williams; Q R Miao Journal: Oncogene Date: 2017-01-09 Impact factor: 9.867