Andras Tomesz1,2, Laszlo Szabo3,2, Richard Molnar3,2, Arpad Deutsch3, Richard Darago3, Domokos Mathe4, Ferenc Budan2,5, Nowrasteh Ghodratollah2, Timea Varjas2, Balazs Nemeth2, Istvan Kiss2. 1. Doctoral School of Health Sciences, Faculty of Health Sciences, University of Pécs, Pécs, Hungary tomesza@gmail.com. 2. Department of Public Health Medicine, Medical School, University of Pécs, Pécs, Hungary. 3. Doctoral School of Health Sciences, Faculty of Health Sciences, University of Pécs, Pécs, Hungary. 4. Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary. 5. Institute of Environmental Engineering, University of Pannonia, Veszprém, Hungary.
Abstract
BACKGROUND/AIM: Development of malignant tumors is preceded by molecular biological events. Our aim was to establish an assay panel by using miRNAs and other genes for the rapid screening of potential carcinogens or chemopreventive agents. MATERIALS AND METHODS: Six male and 6 female CBA/Ca mice received 20 mg/bwkg 7,12-dimethylbenz(α)anthracene (DMBA) intraperitoneally, and 24 h later RNA was isolated from parenchymal organs. Expression of miR-330, miR-29a, miR-9-1, miR-9-3 and mTORC1 was analysed by real time polymerase chain reaction and compared to non-treated controls. RESULTS: DMBA caused significant alterations in the expression of the studied genes. The most profound changes were the strongly elevated miR-9-3 and mTORC1 expressions in female mice in all organs studied. CONCLUSION: miR-9-3 and mTORC1 expression in female mice were found to be the most suitable biomarkers for rapid identification of possible carcinogenic effects. Copyright
BACKGROUND/AIM: Development of malignant tumors is preceded by molecular biological events. Our aim was to establish an assay panel by using miRNAs and other genes for the rapid screening of potential carcinogens or chemopreventive agents. MATERIALS AND METHODS: Six male and 6 female CBA/Ca mice received 20 mg/bwkg 7,12-dimethylbenz(α)anthracene (DMBA) intraperitoneally, and 24 h later RNA was isolated from parenchymal organs. Expression of miR-330, miR-29a, miR-9-1, miR-9-3 and mTORC1 was analysed by real time polymerase chain reaction and compared to non-treated controls. RESULTS:DMBA caused significant alterations in the expression of the studied genes. The most profound changes were the strongly elevated miR-9-3 and mTORC1 expressions in female mice in all organs studied. CONCLUSION:miR-9-3 and mTORC1 expression in female mice were found to be the most suitable biomarkers for rapid identification of possible carcinogenic effects. Copyright
Authors: Ferenc Budán; Tímea Varjas; Ghodratollah Nowrasteh; Ida Prantner; Zsuzsa Varga; Agoston Ember; József Cseh; Katalin Gombos; Emese Pázsit; Gyula Gobel; Miklós Bauer; Tünde Gracza; István Arany; Pál Perjési; István Ember; István Kiss Journal: In Vivo Date: 2009 Jul-Aug Impact factor: 2.155
Authors: Lu Qian Wang; Yok Lam Kwong; Chi Shan Bonnie Kho; Kit Fai Wong; Kwan Yeung Wong; Manuela Ferracin; George A Calin; Chor Sang Chim Journal: Mol Cancer Date: 2013-12-27 Impact factor: 27.401