Chrysoula Kitsou1, Leandros Lazaros1,2, Alexandra Papoudou-Bai3, Prodromos Sakaloglou1, Eirini Mastora1, Theodoros Lykovardakis1, Katerina Giaka1, Georgios Vartholomatos4, Ioanna Bouba1, Sofia Markoula5, Anna Batistatou3, Ioannis Georgiou6. 1. Laboratory of Medical Genetics of Human Reproduction, Medical School, Ioannina University, Ioannina, Greece. 2. Genesis Genoma Lab, Genetic Diagnosis-Clinical Genetics-Research, Athens, Greece. 3. Department of Pathology, Ioannina University Hospital, Ioannina, Greece. 4. Laboratory of Hematology, Molecular Biology Unit, Ioannina University Hospital, Ioannina, Greece. 5. Department of Neurology, Ioannina University Hospital, Ioannina, Greece. 6. Laboratory of Medical Genetics of Human Reproduction, Medical School, Ioannina University, Ioannina, Greece igeorgio@uoi.gr.
Abstract
BACKGROUND/AIM: The expression of reverse transcriptase (RT) in ovaries, testes, gametes and embryos highlights its critical role in cell growth and differentiation. We sought to investigate the effects of the potent RT inhibitor lamivudine in gametogenesis and mouse embryo preimplantation development. MATERIALS AND METHODS: Male and female FVB/N mice were treated with the reverse transcriptase inhibitor Lamivudine for seven consecutive weeks. Following treatment, mouse sperm parameters, testicular and ovarian morphology as well as post-IVF embryo development were evaluated. RESULTS: Lamivudine impaired the sperm parameters and the testicular structure in male mice, the number of primordial germ cells and primary oocytes in ovaries of female mice, and the embryos' morphology and development up to the blastocyst stage during in vitro culture. CONCLUSION: The administration of lamivudine affected the processes of spermatogenesis and oogenesis as well as the in vitro preimplantation development of mouse embryos. Copyright
BACKGROUND/AIM: The expression of reverse transcriptase (RT) in ovaries, testes, gametes and embryos highlights its critical role in cell growth and differentiation. We sought to investigate the effects of the potent RT inhibitor lamivudine in gametogenesis and mouse embryo preimplantation development. MATERIALS AND METHODS: Male and female FVB/N mice were treated with the reverse transcriptase inhibitor Lamivudine for seven consecutive weeks. Following treatment, mouse sperm parameters, testicular and ovarian morphology as well as post-IVF embryo development were evaluated. RESULTS:Lamivudine impaired the sperm parameters and the testicular structure in male mice, the number of primordial germ cells and primary oocytes in ovaries of female mice, and the embryos' morphology and development up to the blastocyst stage during in vitro culture. CONCLUSION: The administration of lamivudine affected the processes of spermatogenesis and oogenesis as well as the in vitro preimplantation development of mouse embryos. Copyright
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