Arnol T Z Tiofack1, Gustave Simo1, Elvis Ofon1, Esther Dina-Bell2, Chancelin M Kamla3, Sidonie N Ananga4, Tchamfong Roger5, Theophile N Nana6, Charlotte T Ngeufack6, Adamou Fewou4,7, Samuel Takongmo7,8, Smiths Lueong1,9. 1. Molecular Parasitology & Entomology Unit, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroun. 2. Medical Oncology, Direction of the Bonassama District Hospital, Douala, Cameroon. 3. Faculty of Medicine and Pharmaceutical Science, University of Douala, Douala, Cameroon. 4. Service of AnatomocytoPathotogy, General Hospital of Douala, Douala, Cameroon. 5. St. Joseph Clinic Cancer Center, Yaounde, Cameroon. 6. Service of Obstetrics and Gynecology, General Hospital of Douala and Faculty of Medicine, University of Buea. 7. Faculty of Medicine and Biomedical Sciences, University of Yaounde, Yaounde, Cameroon. 8. Medico-surgical center of Yaounde-Nsimeyong Hospital, Yaounde, Cameroon. 9. German Cancer Research Center, Essen, Germany.
Abstract
BACKGROUND: Breast cancer (BC) is a leading female cancer worldwide and cause of cancer-related death, especially in developing countries. Genetic predispositions to BC development in African population is poorly studied, and meanwhile the SNP rs17506395 in TP63 gene locus has been associated with the development of breast cancer in Asian women, no investigation has been undertaken within African population. We investigated the impact of this polymorphism in a representative African population. METHODS: We undertook a case-control study including 335 women, of which 111 were breast cancer patients and 224 controls. Using blood-derived germline DNA, PCR-RFLP was used to investigate the polymorphism of TP63 gene at rs17506395 locus. Unconditional logistic regression was used to study the association between the TP63 gene polymorphism and risk of BC development. After stratification into different age and ethno-linguistic groups as well as menopausal status, the Cochran-Mantel-Haenszel test was used to measure significance of the associations. RESULTS: Comparing cases with controls, no significant associations between genotype and disease development was observed. Similarly, when cases were stratified according to menopausal status and ethno-linguistic groups, no significant association was observed between genotype and disease development. However, in women of 40 years and below, TT and TG genotypes were associated with breast cancer development. The minor G allele seems to protective against early breast cancer onset OR of 0.5 (95%CI = 0.26-0.94, p = 0.03). CONCLUSION: Our data revealed an association between rs15706395 and the risk of early breast cancer onset. The GG genotype seems to reduce the risk of early breast cancer. Larger studies are needed to confirm the potential of this SNP as biomarker for breast cancer prognostic.<br />.
BACKGROUND:Breast cancer (BC) is a leading female cancer worldwide and cause of cancer-related death, especially in developing countries. Genetic predispositions to BC development in African population is poorly studied, and meanwhile the SNP rs17506395 in TP63 gene locus has been associated with the development of breast cancer in Asian women, no investigation has been undertaken within African population. We investigated the impact of this polymorphism in a representative African population. METHODS: We undertook a case-control study including 335 women, of which 111 were breast cancerpatients and 224 controls. Using blood-derived germline DNA, PCR-RFLP was used to investigate the polymorphism of TP63 gene at rs17506395 locus. Unconditional logistic regression was used to study the association between the TP63 gene polymorphism and risk of BC development. After stratification into different age and ethno-linguistic groups as well as menopausal status, the Cochran-Mantel-Haenszel test was used to measure significance of the associations. RESULTS: Comparing cases with controls, no significant associations between genotype and disease development was observed. Similarly, when cases were stratified according to menopausal status and ethno-linguistic groups, no significant association was observed between genotype and disease development. However, in women of 40 years and below, TT and TG genotypes were associated with breast cancer development. The minor G allele seems to protective against early breast cancer onset OR of 0.5 (95%CI = 0.26-0.94, p = 0.03). CONCLUSION: Our data revealed an association between rs15706395 and the risk of early breast cancer onset. The GG genotype seems to reduce the risk of early breast cancer. Larger studies are needed to confirm the potential of this SNP as biomarker for breast cancer prognostic.<br />.
Entities:
Keywords:
SNP; TP63; breast cancer; early onset; rs17506395
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