| Literature DB >> 32851098 |
Iole Macchia1, Valentina La Sorsa2, Irene Ruspantini3, Massimo Sanchez3, Valentina Tirelli3, Maria Carollo3, Giorgio Fedele4, Pasqualina Leone4, Giovanna Schiavoni1, Carla Buccione1, Paola Rizza5, Paola Nisticò6, Belinda Palermo6, Stefania Morrone7, Helena Stabile8, Aurelia Rughetti7, Marianna Nuti7, Ilaria Grazia Zizzari7, Cinzia Fionda8, Roberta Maggio9, Cristina Capuano7, Concetta Quintarelli10, Matilde Sinibaldi10, Chiara Agrati11, Rita Casetti11, Andrea Rozo Gonzalez1, Floriana Iacobone1, Angela Gismondi8, Filippo Belardelli1,12, Mauro Biffoni1, Francesca Urbani1,13.
Abstract
[This corrects the article DOI: 10.1155/2020/1938704.].Entities:
Year: 2020 PMID: 32851098 PMCID: PMC7436290 DOI: 10.1155/2020/2698258
Source DB: PubMed Journal: J Immunol Res ISSN: 2314-7156 Impact factor: 4.818
Participants, instruments, and software. Five centres, with a total of 13 operators (including the reference operator, ROP), using seven different flow cytometers dedicated to research use (except GMP-maintained BD FACSCanto™ I at ISS, with a fluidic system upgrade, comparable to a BD FACSCanto™ II), participated to the harmonisation panel. Three flow cytometer models with compatible optical configuration (BC Gallios™, BD FACSCanto™ II, and BD LSR Fortessa™) were used. The data generated were analysed by operators at peripheral sites (local analysis) using their own analysis software (Kaluza, FlowJo, or FACSDiva). Central analysis at ISS was performed by the ROP with Kaluza software on local raw data (acquired fcs files). ∗One of the two BD LSR Fortessa instruments, used by one operator from SUR center, belongs to the core facilities of the CLNS: Center for Life NanoScience@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.
| 5 centres | 13 operators | 7 instruments | 3 instrument models | 2 acquisition software | 3 analysis software |
|---|---|---|---|---|---|
| ISS | 12+1 ROP | 1 FACSCanto I+ | BD FACSCanto II BD LSRFortessa | BC Kaluza | BC Kaluza |