Literature DB >> 32851098

Corrigendum to "Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring".

Iole Macchia1, Valentina La Sorsa2, Irene Ruspantini3, Massimo Sanchez3, Valentina Tirelli3, Maria Carollo3, Giorgio Fedele4, Pasqualina Leone4, Giovanna Schiavoni1, Carla Buccione1, Paola Rizza5, Paola Nisticò6, Belinda Palermo6, Stefania Morrone7, Helena Stabile8, Aurelia Rughetti7, Marianna Nuti7, Ilaria Grazia Zizzari7, Cinzia Fionda8, Roberta Maggio9, Cristina Capuano7, Concetta Quintarelli10, Matilde Sinibaldi10, Chiara Agrati11, Rita Casetti11, Andrea Rozo Gonzalez1, Floriana Iacobone1, Angela Gismondi8, Filippo Belardelli1,12, Mauro Biffoni1, Francesca Urbani1,13.   

Abstract

[This corrects the article DOI: 10.1155/2020/1938704.].
Copyright © 2020 Iole Macchia et al.

Entities:  

Year:  2020        PMID: 32851098      PMCID: PMC7436290          DOI: 10.1155/2020/2698258

Source DB:  PubMed          Journal:  J Immunol Res        ISSN: 2314-7156            Impact factor:   4.818


In the article titled “Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring” [1], there was an error in Table 1. The table should show affiliation of one of the two BD LSR Fortessa instruments used in the study and cited in Table 1. The corrected table is shown below and is listed as Table 1.
Table 1

Participants, instruments, and software. Five centres, with a total of 13 operators (including the reference operator, ROP), using seven different flow cytometers dedicated to research use (except GMP-maintained BD FACSCanto™ I at ISS, with a fluidic system upgrade, comparable to a BD FACSCanto™ II), participated to the harmonisation panel. Three flow cytometer models with compatible optical configuration (BC Gallios™, BD FACSCanto™ II, and BD LSR Fortessa™) were used. The data generated were analysed by operators at peripheral sites (local analysis) using their own analysis software (Kaluza, FlowJo, or FACSDiva). Central analysis at ISS was performed by the ROP with Kaluza software on local raw data (acquired fcs files). ∗One of the two BD LSR Fortessa instruments, used by one operator from SUR center, belongs to the core facilities of the CLNS: Center for Life NanoScience@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.

5 centres13 operators7 instruments3 instrument models2 acquisition software3 analysis software
ISSSURINMIIREOPBG12+1 ROP1 FACSCanto I+(GMP)3 BD FACSCanto II2 BD LSRFortessa1 BC GalliosBD FACSCanto II BD LSRFortessaBC GalliosBC KaluzaBD FACSDivaBC KaluzaBD DivaTreeStarFlowJo
  1 in total

1.  Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring.

Authors:  Iole Macchia; Valentina La Sorsa; Irene Ruspantini; Massimo Sanchez; Valentina Tirelli; Maria Carollo; Giorgio Fedele; Pasqualina Leone; Giovanna Schiavoni; Carla Buccione; Paola Rizza; Paola Nisticò; Belinda Palermo; Stefania Morrone; Helena Stabile; Aurelia Rughetti; Marianna Nuti; Ilaria Grazia Zizzari; Cinzia Fionda; Roberta Maggio; Cristina Capuano; Concetta Quintarelli; Matilde Sinibaldi; Chiara Agrati; Rita Casetti; Andrea Rozo Gonzalez; Floriana Iacobone; Angela Gismondi; Filippo Belardelli; Mauro Biffoni; Francesca Urbani
Journal:  J Immunol Res       Date:  2020-04-12       Impact factor: 4.818

  1 in total

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