Bijan Khademi1,2, Sima Safari2, Mohammad Amin Mosleh-Shirazi3, Maral Mokhtari4, Nooshafarin Chenari5, Mahboobeh Razmkhah5. 1. Research Center of Otolaryngology Head and Neck Surgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Department of Otolaryngology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Department of Radiotherapy and Oncology, Medical Imaging Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 5. Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
BACKGROUND: Radiation-induced skin injury remains a serious concern, which may limit the duration and dose of radiation treatment. The concept that stem cell injection may reduce tissue injury or assist its recovery after radiation has been recently argued. Herein, we examined the effect of adipose-derived mesenchymal stem cells (ASCs) on radiation-induced skin damage in rats. METHODS: This study is an experimental case control study. ASCs were isolated from peri uterine fat tissue of the rats. Then the rats received a 30 Gy single dose radiation to their buttocks skin using gamma radiation. Next day stem cells were transplanted subcutaneously in 16 rats as the case group. A group of 16 rats was considered as control group with radiation but no transplantation of stem cells. Then rats were examined and observed by macroscopic analysis and phenotypic scores during 4 weeks of follow up. RESULTS: The wound size in control group was significantly higher than case group in the second, third and fourth weeks of evaluation (P<0.05). There was no significant difference in skin lesion severity, pathological factors, and the onset of recovery signs between two groups (P>0.05). CONCLUSIONS: It seems that using ASCs alone has not profound effects on reducing radiation-induced cutaneous complications, while combination of these cells with growth factors may produce more promising results. 2020 Stem Cell Investigation. All rights reserved.
BACKGROUND: Radiation-induced skin injury remains a serious concern, which may limit the duration and dose of radiation treatment. The concept that stem cell injection may reduce tissue injury or assist its recovery after radiation has been recently argued. Herein, we examined the effect of adipose-derived mesenchymal stem cells (ASCs) on radiation-induced skin damage in rats. METHODS: This study is an experimental case control study. ASCs were isolated from peri uterine fat tissue of the rats. Then the rats received a 30 Gy single dose radiation to their buttocks skin using gamma radiation. Next day stem cells were transplanted subcutaneously in 16 rats as the case group. A group of 16 rats was considered as control group with radiation but no transplantation of stem cells. Then rats were examined and observed by macroscopic analysis and phenotypic scores during 4 weeks of follow up. RESULTS: The wound size in control group was significantly higher than case group in the second, third and fourth weeks of evaluation (P<0.05). There was no significant difference in skin lesion severity, pathological factors, and the onset of recovery signs between two groups (P>0.05). CONCLUSIONS: It seems that using ASCs alone has not profound effects on reducing radiation-induced cutaneous complications, while combination of these cells with growth factors may produce more promising results. 2020 Stem Cell Investigation. All rights reserved.
Authors: Ivan Hadad; Brian H Johnstone; Jeffrey G Brabham; Matthew W Blanton; Pamela I Rogers; Cory Fellers; James L Solomon; Stephanie Merfeld-Clauss; Colleen M DesRosiers; Joseph R Dynlacht; John J Coleman; Keith L March Journal: Int J Radiat Oncol Biol Phys Date: 2010-08-12 Impact factor: 7.038