Sujan Khadka1,2,3, Sanjeep Sapkota1,4,3, Sanjib Adhikari5, Ashok Kumar Dubey1, Alina Thapa6,3, Rajendra Bashyal7, Hari Bhusal8. 1. Department of Microbiology, Birendra Multiple Campus, Tribhuvan University, Chitwan, Bharatpur, 44200, Nepal. 2. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. 3. University of Chinese Academy of Sciences, Beijing, 100049, China. 4. State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. 5. Department of Microbiology, Birendra Multiple Campus, Tribhuvan University, Chitwan, Bharatpur, 44200, Nepal. sanadh26@gmail.com. 6. State Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 100101, China. 7. Department of Anatomy, Physiology and Biochemistry, Agriculture and Forestry University, Rampur, 44209, Nepal. 8. Seed Medical Laboratory, Chitwan, Bharatpur, 44200, Nepal.
Abstract
BACKGROUND: Intestinal parasitosis is one of the commonly perceived serious problems often observed in children leading to high mortality. The objective of the study was to identify the intestinal parasites and study their prevalence in the two mostly disadvantaged communities (Musahar and Chepang) of Nepal. METHODS: This was a cross-sectional study conducted in the Musahar and Chepang communities of Nepal from April to October 2019. A total of 205 random stool samples were collected in dry, clean and screw-capped plastic containers and mixed with 2.5% potassium dichromate solution. A pre-structured questionnaire was used to collect data on predisposing factors. The laboratory examination of the stool samples was done by direct microscopy and further confirmed by concentration methods (formalin ether sedimentation technique and flotation technique using Sheather's sugar solution), and modified acid-fast staining. Detection of eggs of Enterobius vermicularis was done by cellophane tape method. RESULTS: The overall prevalence of intestinal parasitic infection was found to be 36.6%, with a similar prevalence in the Chepangs (39.8%) and in the Musahars (33.3%) (P > 0.05). The most predominant helminth was Ascaris lumbricoides (15.6%), while the most prevalent protozoan was Entamoeba histolytica/dispar (5.4%). The study also assessed a significant association between the prevalence of parasites with socio-demographic factors, types of drinking water consumption and sanitation habits of the people (P < 0.05). CONCLUSION: The findings of the study suggest a need for formulating effective preventive and control strategies against intestinal parasitic infections along with the continuity of mass deworming program.
BACKGROUND: Intestinal parasitosis is one of the commonly perceived serious problems often observed in children leading to high mortality. The objective of the study was to identify the intestinal parasites and study their prevalence in the two mostly disadvantaged communities (Musahar and Chepang) of Nepal. METHODS: This was a cross-sectional study conducted in the Musahar and Chepang communities of Nepal from April to October 2019. A total of 205 random stool samples were collected in dry, clean and screw-capped plastic containers and mixed with 2.5% potassium dichromate solution. A pre-structured questionnaire was used to collect data on predisposing factors. The laboratory examination of the stool samples was done by direct microscopy and further confirmed by concentration methods (formalin ether sedimentation technique and flotation technique using Sheather's sugar solution), and modified acid-fast staining. Detection of eggs of Enterobius vermicularis was done by cellophane tape method. RESULTS: The overall prevalence of intestinal parasitic infection was found to be 36.6%, with a similar prevalence in the Chepangs (39.8%) and in the Musahars (33.3%) (P > 0.05). The most predominant helminth was Ascaris lumbricoides (15.6%), while the most prevalent protozoan was Entamoeba histolytica/dispar (5.4%). The study also assessed a significant association between the prevalence of parasites with socio-demographic factors, types of drinking water consumption and sanitation habits of the people (P < 0.05). CONCLUSION: The findings of the study suggest a need for formulating effective preventive and control strategies against intestinal parasitic infections along with the continuity of mass deworming program.
Entities:
Keywords:
Chepang; Concentration methods; Deworming; Direct microscopy; Intestinal parasitosis; Musahar
Authors: Batu Krishna Sharma; Shiba Kumar Rai; Diyo Ram Rai; Deepali Roy Choudhury Journal: Southeast Asian J Trop Med Public Health Date: 2004-09 Impact factor: 0.267
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