| Literature DB >> 3282909 |
Abstract
More than 99% of L. lactis cells have been converted to protoplasts upon digestion of cell walls with mutanolysin (N-(acetyl)muramidase). Functional protoplasts were obtained even with the lowest level of the enzyme that was used (0.1 U.ml-1 of the cell suspension) and after incubation at 37 degrees C for 2 min. The regeneration of the polymerized cell wall appears to be induced by a cell homogenate of the same organism.Entities:
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Year: 1988 PMID: 3282909 DOI: 10.1007/bf01961279
Source DB: PubMed Journal: Experientia ISSN: 0014-4754