Jacob S Suissa1,2, Walton A Green1. 1. Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA. 2. Arnold Arboretum of Harvard University, Boston, MA, USA.
Abstract
BACKGROUND AND AIMS: Crassulacean acid metabolism (CAM) is an adaptation to increase water use efficiency in dry environments. Similar biochemical patterns occur in the aquatic lycophyte genus Isoëtes. It has long been assumed and accepted that CAM-like behaviour in these aquatic plants is an adaptation to low daytime carbon levels in aquatic ecosystems, but this has never been directly tested. METHODS: To test this hypothesis, populations of Isoëtes engelmannii and I. tuckermanii were grown in climate-controlled chambers and starved of atmospheric CO2 during the day while pH was measured for 24 h. KEY RESULTS: We demonstrate that terrestrial plants exposed to low atmospheric CO2 display diel acidity cycles similar to those in both xerophytic CAM plants and submerged Isoëtes. CONCLUSIONS: Daytime CO2 starvation induces CAM-like nocturnal acid accumulation in terrestrial Isoëtes, substantiating the hypothesis that carbon starvation is a selective pressure for this physiological behaviour.
BACKGROUND AND AIMS: Crassulacean acid metabolism (CAM) is an adaptation to increase water use efficiency in dry environments. Similar biochemical patterns occur in the aquatic lycophyte genus Isoëtes. It has long been assumed and accepted that CAM-like behaviour in these aquatic plants is an adaptation to low daytime carbon levels in aquatic ecosystems, but this has never been directly tested. METHODS: To test this hypothesis, populations of Isoëtes engelmannii and I. tuckermanii were grown in climate-controlled chambers and starved of atmospheric CO2 during the day while pH was measured for 24 h. KEY RESULTS: We demonstrate that terrestrial plants exposed to low atmospheric CO2 display diel acidity cycles similar to those in both xerophytic CAM plants and submerged Isoëtes. CONCLUSIONS: Daytime CO2 starvation induces CAM-like nocturnal acid accumulation in terrestrial Isoëtes, substantiating the hypothesis that carbon starvation is a selective pressure for this physiological behaviour.
Authors: Douwe G Van Der Meer; Richard E Zeebe; Douwe J J van Hinsbergen; Appy Sluijs; Wim Spakman; Trond H Torsvik Journal: Proc Natl Acad Sci U S A Date: 2014-03-10 Impact factor: 11.205
Authors: David Wickell; Li-Yaung Kuo; Hsiao-Pei Yang; Amra Dhabalia Ashok; Iker Irisarri; Armin Dadras; Sophie de Vries; Jan de Vries; Yao-Moan Huang; Zheng Li; Michael S Barker; Nolan T Hartwick; Todd P Michael; Fay-Wei Li Journal: Nat Commun Date: 2021-11-03 Impact factor: 14.919