Hanjun Chen1, Zhaoyun Li1, Lihong Zhang2, Liming Zhang1, Yaqiong Zhang1, Yichao Wang1, Meifen Xu1, Qianyi Zhong3. 1. Department of Clinical Laboratory, Taizhou Central Hospital (Taizhou University Hospital), Taizhou. 2. Department of Clinical Laboratory Center, Shaoxing People's Hospital, Shaoxing, China. 3. Department of Clinical Laboratory, Taizhou Central Hospital (Taizhou University Hospital), Taizhou 13606927095@163.com.
Abstract
OBJECTIVE: Triple-negative breast cancer (TNBC) is one of the most common malignant, highly heterogeneous tumors in women. MicroRNAs (miRNAs), such as miR-200c, play an important role in various types of malignant cancer, including TNBC. However, the biological role of miRNA-200c in TNBC is not well understood. In this study, we investigated the mechanism of miR-200c in the growth of TNBC. METHODS: Reverse transcription quantitative polymerase chain reaction was used to detect the expression of miR-200c in TNBC tissues and TNBC cells. Cell Counting Kit-8 (CCK-8) assays, wound healing, and transwell assays were used to observe the effects of miR-200c on TNBC cell proliferation, migration, and invasion, respectively. The expression of epithelial-mesenchymal transition (EMT) markers were detected by Western blotting. Dual luciferase reporter assays were used to test whether ZEB2 is a novel target of miR-200c. RESULT: Our results show that ZEB2 is a novel target of miR-200c and that ZEB2 mediates the metastasis of triple-negative breast cancer via EMT. CONCLUSION: miR-200c attenuates TNBC cell invasion and EMT by targeting ZEB2. Our data therefore suggest that miR-200c may be used to develop novel early-stage diagnostic and therapeutic strategies for TNBC.
OBJECTIVE: Triple-negative breast cancer (TNBC) is one of the most common malignant, highly heterogeneous tumors in women. MicroRNAs (miRNAs), such as miR-200c, play an important role in various types of malignant cancer, including TNBC. However, the biological role of miRNA-200c in TNBC is not well understood. In this study, we investigated the mechanism of miR-200c in the growth of TNBC. METHODS: Reverse transcription quantitative polymerase chain reaction was used to detect the expression of miR-200c in TNBC tissues and TNBC cells. Cell Counting Kit-8 (CCK-8) assays, wound healing, and transwell assays were used to observe the effects of miR-200c on TNBC cell proliferation, migration, and invasion, respectively. The expression of epithelial-mesenchymal transition (EMT) markers were detected by Western blotting. Dual luciferase reporter assays were used to test whether ZEB2 is a novel target of miR-200c. RESULT: Our results show that ZEB2 is a novel target of miR-200c and that ZEB2 mediates the metastasis of triple-negative breast cancer via EMT. CONCLUSION:miR-200c attenuates TNBC cell invasion and EMT by targeting ZEB2. Our data therefore suggest that miR-200c may be used to develop novel early-stage diagnostic and therapeutic strategies for TNBC.
Authors: Lenka Kalinkova; Nataliia Nikolaieva; Bozena Smolkova; Sona Ciernikova; Karol Kajo; Vladimir Bella; Viera Horvathova Kajabova; Helena Kosnacova; Gabriel Minarik; Ivana Fridrichova Journal: Int J Mol Sci Date: 2021-12-22 Impact factor: 5.923