Literature DB >> 32822022

Detection of Modified Bases in Bacteriophage Genomic DNA.

Yan-Jiun Lee1, Peter R Weigele2.   

Abstract

Collectively, the dsDNA tailed bacteriophages (Caudovirales) contain the largest chemical diversity of naturally occurring deoxynucleotides in DNA observed to date. The continuing discovery of new modifications in phages suggest many more are waiting to be found. Thus, methods for the observation and characterization of noncanonical nucleosides are timely. We present here protocols for extraction of genomic DNA from bacteriophage particles, enzymatic hydrolysis of DNA to free nucleosides, and examination of nucleoside composition by HPLC and mass spectrometry.

Entities:  

Keywords:  Bacteriophages; DNA modifications; HPLC; Mass spectrometry

Mesh：

Substances：

Year:  2021        PMID： 32822022     DOI： 10.1007/978-1-0716-0876-0_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Binding Affinity Quantifications of the Bacteriophage Mu DNA Modification Protein Mom Using Microscale Thermophoresis (MST).

Authors:  Shubha Udupa; Valakunja Nagaraja; Shweta Karambelkar
Journal:  Bio Protoc       Date:  2022-07-20

2.  Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design.

Authors:  John M Pryor; Vladimir Potapov; Katharina Bilotti; Nilisha Pokhrel; Gregory J S Lohman
Journal:  ACS Synth Biol       Date:  2022-05-25       Impact factor: 5.249

3.  Pathways of thymidine hypermodification.

Authors:  Yan-Jiun Lee; Nan Dai; Stephanie I Müller; Chudi Guan; Mackenzie J Parker; Morgan E Fraser; Shannon E Walsh; Janani Sridar; Andrew Mulholland; Krutika Nayak; Zhiyi Sun; Yu-Cheng Lin; Donald G Comb; Katherine Marks; Reyaz Gonzalez; Daniel P Dowling; Vahe Bandarian; Lana Saleh; Ivan R Corrêa; Peter R Weigele
Journal:  Nucleic Acids Res       Date:  2022-04-08       Impact factor: 16.971
  3 in total

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