Mike Stern1, Melanie Giebels2, Tilman Fey2, Margit Lübking3, Judith Alferink3, Georg Hempel1. 1. Institut für Pharmazeutische und Medizinische Chemie - Klinische Pharmazie, Westfälische Wilhelms-Universität, Corrensstraße 48, 48149 Münster, Germany. 2. Gerontopsychiatrie, LWL-Klinik Münster, Friedrich-Wilhelm-Weber-Straße 30, 48147 Münster, Germany. 3. Damian-Klinik, Alexianer Münster GmbH, Alexianerweg 9, 48163 Münster, Germany.
Abstract
Aim: Volumetric absorptive microsampling offers a hematocrit insensitive way for microsampling. The aim of this study was to develop a method for routine healthcare for 14 antidepressants, antipsychotics and their active metabolites on a single-quadrupole HPLC-MS. A clinical validation study to determine conversion factors from capillary blood to plasma concentration was conducted afterward. Results: The method was validated according to current guidelines except for one substance. Five substances were measured in 49 patient samples and conversion factors could be derived with Passing-Bablok and Bland-Altman analysis. Conclusion: A reliable extraction and analysis method for commonly used antidepressants and antipsychotics was developed and validated. The method with the obtained conversion factors can now be used in routine healthcare.
Aim: Volumetric absorptive microsampling offers a hematocrit insensitive way for microsampling. The aim of this study was to develop a method for routine healthcare for 14 antidepressants, antipsychotics and their active metabolites on a single-quadrupole HPLC-MS. A clinical validation study to determine conversion factors from capillary blood to plasma concentration was conducted afterward. Results: The method was validated according to current guidelines except for one substance. Five substances were measured in 49 patient samples and conversion factors could be derived with Passing-Bablok and Bland-Altman analysis. Conclusion: A reliable extraction and analysis method for commonly used antidepressants and antipsychotics was developed and validated. The method with the obtained conversion factors can now be used in routine healthcare.
Authors: Anna M Mc Laughlin; Eduard Schmulenson; Olga Teplytska; Sebastian Zimmermann; Patrick Opitz; Stefanie L Groenland; Alwin D R Huitema; Neeltje Steeghs; Lothar Müller; Stefan Fuxius; Gerald Illerhaus; Markus Joerger; Frank Mayer; Uwe Fuhr; Stefan Holdenrieder; Georg Hempel; Oliver Scherf-Clavel; Ulrich Jaehde; Charlotte Kloft Journal: Cancers (Basel) Date: 2021-12-14 Impact factor: 6.639