Ferenc Torma1, Zoltan Gombos1, Marcell Fridvalszki2, Gergely Langmar2, Zsofia Tarcza3, Bela Merkely3, Hisashi Naito4, Noriko Ichinoseki-Sekine4, Masaki Takeda5, Zsolt Murlasits6, Peter Osvath7, Zsolt Radak8. 1. Research Center for Molecular Exercise Science, University of Physical Education, Budapest 1123, Hungary. 2. Department of Kinesiology, University of Physical Education, Budapest 1123, Hungary. 3. Heart and Vascular Center, Semmelweis University, Budapest 1122, Hungary. 4. Faculty of Health and Sports Science, Juntendo University, Chiba 270-1695, Japan. 5. Faculty of Health and Sports Science, Doshisha University, Kyotanabe 610-0394, Japan. 6. Laboratory of Animal Research Center, Qatar University, Doha 2713, Qatar. 7. Department of Health Sciences and Sport Medicine, University of Physical Education, Budapest 1123, Hungary. 8. Research Center for Molecular Exercise Science, University of Physical Education, Budapest 1123, Hungary. Electronic address: radak.zsolt@tf.hu.
Abstract
BACKGROUD: Blood flow restriction (BFR) with low-intensity resistance training has been shown to result in hypertrophy of skeletal muscle. In this study, we tested the hypothesis that BFR during the rest periods between acute, high-intensity resistance exercise sessions (70% of 1 repetition maximum, 7 sets with 10 repetitions) enhances the effects of the resistance training. METHODS: A total of 7 healthy young men performed squats, and between sets BFR was carried out on one leg while the other leg served as a control. Because BFR was applied during rest periods, even severe occlusion pressure (approximately 230 mmHg), which almost completely blocked blood flow, was well-tolerated by the participants. Five muscle-specific microRNAs were measured from the biopsy samples, which were taken 2 h after the acute training. RESULTS: Doppler data showed that the pattern of blood flow recovery changed significantly between the first and last BFR. microRNA-206 levels significantly decreased in the BFR leg compared to the control. The mRNA levels of RAC-β serine/threonine-protein kinase v22, nuclear respiratory factor 1, vascular endothelial growth factor, lupus Ku autoantigen protein p70 genes (p < 0.05), and paired box 7 (p < 0.01) increased in the BFR leg. The protein levels of paired box 7, nuclear respiratory factor 1, and peroxisome proliferator-activated receptor γ coactivator 1α did not differ between the BFR leg and the control leg. CONCLUSION: BFR, during the rest periods of high-load resistance training, could lead to mRNA elevation of those proteins that regulate angiogenesis, mitochondrial biogenesis, and muscle hypertrophy and repair. However, BFR also can cause DNA damage, judging from the increase in mRNA levels of lupus Ku autoantigen protein p70.
BACKGROUD: Blood flow restriction (BFR) with low-intensity resistance training has been shown to result in hypertrophy of skeletal muscle. In this study, we tested the hypothesis that BFR during the rest periods between acute, high-intensity resistance exercise sessions (70% of 1 repetition maximum, 7 sets with 10 repetitions) enhances the effects of the resistance training. METHODS: A total of 7 healthy young men performed squats, and between sets BFR was carried out on one leg while the other leg served as a control. Because BFR was applied during rest periods, even severe occlusion pressure (approximately 230 mmHg), which almost completely blocked blood flow, was well-tolerated by the participants. Five muscle-specific microRNAs were measured from the biopsy samples, which were taken 2 h after the acute training. RESULTS: Doppler data showed that the pattern of blood flow recovery changed significantly between the first and last BFR. microRNA-206 levels significantly decreased in the BFR leg compared to the control. The mRNA levels of RAC-β serine/threonine-protein kinase v22, nuclear respiratory factor 1, vascular endothelial growth factor, lupus Ku autoantigen protein p70 genes (p < 0.05), and paired box 7 (p < 0.01) increased in the BFR leg. The protein levels of paired box 7, nuclear respiratory factor 1, and peroxisome proliferator-activated receptor γ coactivator 1α did not differ between the BFR leg and the control leg. CONCLUSION: BFR, during the rest periods of high-load resistance training, could lead to mRNA elevation of those proteins that regulate angiogenesis, mitochondrial biogenesis, and muscle hypertrophy and repair. However, BFR also can cause DNA damage, judging from the increase in mRNA levels of lupus Ku autoantigen protein p70.
Authors: Robert Trybulski; Jakub Jarosz; Michal Krzysztofik; Milena Lachowicz; Grzegorz Trybek; Adam Zajac; Michal Wilk Journal: Sci Rep Date: 2022-04-08 Impact factor: 4.379
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Authors: Krzysztof Fostiak; Marta Bichowska; Robert Trybulski; Bartosz Trabka; Michal Krzysztofik; Nicholas Rolnick; Aleksandra Filip-Stachnik; Michal Wilk Journal: Int J Environ Res Public Health Date: 2022-10-03 Impact factor: 4.614