Literature DB >> 32808108

Rapid identification of fungi from respiratory samples by Bruker Biotyper matrix-assisted laser desorption/ionisation time-of-flight using ID-FUNGI plates.

Emilie Cardot Martin1, Claudine Renaux2, Emilie Catherinot3, Lucie Limousin2, Louis Jean Couderc3,4, Marc Vasse2.   

Abstract

Identification of moulds is crucial for the clinical management of patients. The goal of this study was to evaluate the new ID-FUNGI plate (IDFP) for the identification of moulds by MALDI Biotyper. IDFP was compared with Sabouraud with gentamicin and chloramphenicol plate (SAB) for the identification of 80 moulds from respiratory samples and eight reference strains. With the direct transfer method, species identification rose from 6% with SAB to 68% with IDFP using score cut-off 2 and from 20 to 75% using cut-off 1.7 (p < 0.001). Our study highlights that the new IDFP improves mycological diagnostic and workflow in laboratories.

Entities:  

Keywords:  Bruker; Fungi; ID-FUNGI plate; MALDI TOF MS

Mesh：

Year:  2020        PMID： 32808108     DOI： 10.1007/s10096-020-04007-2

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  9 in total

Review 1.  The global problem of antifungal resistance: prevalence, mechanisms, and management.

Authors:  David S Perlin; Riina Rautemaa-Richardson; Ana Alastruey-Izquierdo
Journal:  Lancet Infect Dis       Date:  2017-07-31       Impact factor: 25.071

Review 2.  Antifungal prophylaxis during neutropenia and immunodeficiency.

Authors:  O Lortholary; B Dupont
Journal:  Clin Microbiol Rev       Date:  1997-07       Impact factor: 26.132

Review 3.  Identification of Molds by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.

Authors:  Maurizio Sanguinetti; Brunella Posteraro
Journal:  J Clin Microbiol       Date:  2016-11-02       Impact factor: 5.948

4.  Use of the Bruker MALDI Biotyper for identification of molds in the clinical mycology laboratory.

Authors:  Bettina Schulthess; Raphael Ledermann; Forouhar Mouttet; Andrea Zbinden; Guido V Bloemberg; Erik C Böttger; Michael Hombach
Journal:  J Clin Microbiol       Date:  2014-05-21       Impact factor: 5.948

5.  Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Aspergillus, Scedosporium, and Fusarium spp. in the Australian Clinical Setting.

Authors:  Sue Sleiman; Catriona L Halliday; Belinda Chapman; Mitchell Brown; Joanne Nitschke; Anna F Lau; Sharon C-A Chen
Journal:  J Clin Microbiol       Date:  2016-06-01       Impact factor: 5.948

Review 6.  Diagnosis and treatment of invasive pulmonary aspergillosis in neutropenic patients.

Authors:  F Reichenberger; J M Habicht; A Gratwohl; M Tamm
Journal:  Eur Respir J       Date:  2002-04       Impact factor: 16.671

7.  Polyphasic taxonomy of Aspergillus section Aspergillus (formerly Eurotium), and its occurrence in indoor environments and food.

Authors:  A J Chen; V Hubka; J C Frisvad; C M Visagie; J Houbraken; M Meijer; J Varga; R Demirel; Ž Jurjević; A Kubátová; F Sklenář; Y G Zhou; R A Samson
Journal:  Stud Mycol       Date:  2017-07-12       Impact factor: 16.097

8.  Matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and accurate identification of Pseudallescheria/Scedosporium species.

Authors:  E Sitterlé; S Giraud; J Leto; J P Bouchara; A Rougeron; F Morio; B Dauphin; C Angebault; G Quesne; J L Beretti; N Hassouni; X Nassif; M E Bougnoux
Journal:  Clin Microbiol Infect       Date:  2014-02-28       Impact factor: 8.067

9.  Mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

Authors:  Carole Cassagne; Stéphane Ranque; Anne-Cécile Normand; Patrick Fourquet; Sandrine Thiebault; Chantal Planard; Marijke Hendrickx; Renaud Piarroux
Journal:  PLoS One       Date:  2011-12-14       Impact factor: 3.240
  9 in total
  3 in total

Review 1.  Matrix-Assisted Laser Desorption Ionization Time-of-Flight for Fungal Identification.

Authors:  Anna F Lau
Journal:  Clin Lab Med       Date:  2021-04-24       Impact factor: 2.172

2.  A New Filter Based Cultivation Approach for Improving Aspergillus Identification using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS).

Authors:  Husam Salah; Anna Kolecka; Anna Rozaliyani; Retno Wahyuningsih; Saad J Taj-Aldeen; Teun Boekhout; Jos Houbraken
Journal:  Mycopathologia       Date:  2022-01-10       Impact factor: 2.574

Review 3.  MALDI-TOF MS in a Medical Mycology Laboratory: On Stage and Backstage.

Authors:  Marie-Gladys Robert; Muriel Cornet; Aurélie Hennebique; Tahinamandranto Rasamoelina; Yvan Caspar; Léa Pondérand; Marie Bidart; Harmonie Durand; Marvin Jacquet; Cécile Garnaud; Danièle Maubon
Journal:  Microorganisms       Date:  2021-06-12
  3 in total

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