| Literature DB >> 32807016 |
Reza Mahmoudi1, Seyedeh Ashraf Mirahmadi-Babaheidri2, Hamdollah Delaviz1, Mohamad Hassan Fouani3, Mohsen Alipour4, Mehrzad Jafari Barmak1, Gunna Christiansen5, Hassan Bardania1,6,7.
Abstract
In this study, turmeric's active ingredient (Curcumin) was encapsulated into RGD modified Liposomes (RGD-Lip-Cur) its cytotoxic effect on the breast cancer cell line (MCF-7) was evaluated by MTT, flow cytometry and Caspase assay. Liposomes were characterized using transmission electron microscopy (TEM). Results demonstrated that the liposomes were spherical in shape, ranging from 70 to 100 nm. MTT assay revealed that RGD-Lip-Cur had a significant cytotoxic effect on MCF-7 cells at concentrations of 32, 16 and 4 μg/ml compared to Lip-Cur (P < 0.05) and curcumin (P < 0.01). The apoptosis assay demonstrated that RGD-Lip-Cur induces the apoptosis in MCF-7 cells (39.6% vs 40.2% for initial and secondary apoptosis) significantly more than Lip-Cur (67.7% vs 9.16% for initial and secondary apoptosis) and free curcumin (7.84% vs 38.8% for initial and secondary apoptosis). Moreover, caspase assay showed that RGD-Lip-Cur activates caspase 3/7 compared to Lip-Cur (P < 0.05) and free curcumin (P < 0.01). The RGD-Lip-Cur was similar to the control group and had no significant cytotoxicity effect. It is concluded that RGD-Lip-Cur as a novel carrier have high cytotoxicity effect on breast cancer cell line (MCF-7).Entities:
Keywords: RGD; RGD-modified liposome; apoptosis assay; curcumin; liposome
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Year: 2020 PMID： 32807016 DOI： 10.1177/0885328220949367
Source DB: PubMed Journal: J Biomater Appl ISSN： 0885-3282 Impact factor: 2.646