Resorbable bacterial cellulose membranes with strontium release for guided bone regeneration.

Hybrid materials, based on bacterial cellulose (BC) and hydroxyapatite (HA), have been investigated for guided bone regeneration (GBR). However, for some GBR, degradability in the physiological environment is an essential requirement. The present study aimed to explore the use of oxidized bacterial cellulose (OxBC) membranes, associated with strontium apatite, for GBR applications. BC membranes were produced by fermentation and purified, before oxidizing and mineralizing by immersing in strontium chloride solution and sodium bibasic phosphate for 5 cycles. The hybrid materials (BC/HA/Sr, BC/SrAp, OxBC/HA/Sr and OxBC/SrAp) were characterized for biodegradability and bioactivity and for their physicochemical and morphological properties. In vitro cytotoxicity and hemolytic properties of the materials were also investigated. In vivo biocompatibility was analyzed by performing histopathological evaluation at 1, 3 and 9 weeks in mices. Results showed that the samples presented different strontium release profiles and that oxidation enhances degradation under physiological conditions. All the hybrid materials were bioactive. Cell viability assay indicated that the materials are non-cytotoxic and in vivo studies showed low inflammatory response and increased connective tissue repair, as well as degradation in most of the materials, especially the oxidized membranes. This study confirms the potential use of bacterial cellulose-derived hybrid membranes for GBR.