Literature DB >> 3279419

Nucleotide sequence for yeast dihydrolipoamide dehydrogenase.

K S Browning1, D J Uhlinger, L J Reed.   

Abstract

Rabbit antiserum to the dihydrolipoamide dehydrogenase (dihydrolipoamide:NAD+ oxidoreductase, EC 1.8.1.4) component of the pyruvate dehydrogenase complex from bakers' yeast was used to screen plaques produced by a lambda gt11 yeast cDNA library. A 2.1-kilobase insert was isolated that also hybridized to a 17-base mixed oligonucleotide probe corresponding to the amino-terminal sequence of the yeast dihydrolipoamide dehydrogenase. The cDNA has a coding sequence of 499 amino acids that corresponds to a 21-residue signal peptide and a 478-residue mature protein (Mr = 51,558). Computer analysis shows that yeast dihydrolipoamide dehydrogenase has about 41% amino acid identity with Escherichia coli dihydrolipoamide dehydrogenase. Particularly striking is the conservation of sequence in the active site region of the dihydrolipoamide dehydrogenases from E. coli, yeast, and pig heart.

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Year:  1988        PMID： 3279419      PMCID： PMC279873          DOI： 10.1073/pnas.85.6.1831

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

1.  An amino acid sequence in the active site of lipoamide dehydrogenase from the 2-oxoglutarate dehydrogenase complex of E. coli (Crookes strain).

Authors:  J P Brown; R N Perham
Journal:  FEBS Lett       Date:  1972-10-01       Impact factor: 4.124

2.  The isolation and primary structure of a paptide containing the oxidation-reduction active cystine of Escherichia coli lipoamide dehydrogenase.

Authors:  B D Burleigh; C H Williams
Journal:  J Biol Chem       Date:  1972-04-10       Impact factor: 5.157

3.  Lipoyl dehydrogenase from Saccharomyces cerevisiae. I. Purification and some properties.

Authors:  A Wren; V Massey
Journal:  Biochim Biophys Acta       Date:  1965-11-22

4.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

5.  Glutathione reductase from Escherichia coli: mutation, cloning and sequence analysis of the gene.

Authors:  R N Perham
Journal:  Biochem Soc Trans       Date:  1987-08       Impact factor: 5.407

6.  Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12.

Authors:  P E Stephens; H M Lewis; M G Darlison; J R Guest
Journal:  Eur J Biochem       Date:  1983-10-03

7.  Amino acid sequence homology between pig heart lipoamide dehydrogenase and human erythrocyte glutathione reductase.

Authors:  C H Williams; L D Arscott; G E Schulz
Journal:  Proc Natl Acad Sci U S A       Date:  1982-04       Impact factor: 11.205

8.  Glutathione reductase from human erythrocytes. The sequences of the NADPH domain and of the interface domain.

Authors:  R L Krauth-Siegel; R Blatterspiel; M Saleh; E Schiltz; R H Schirmer; R Untucht-Grau
Journal:  Eur J Biochem       Date:  1982-01

9.  Nucleotide sequence of a gene from the Pseudomonas transposon Tn501 encoding mercuric reductase.

Authors:  N L Brown; S J Ford; R D Pridmore; D C Fritzinger
Journal:  Biochemistry       Date:  1983-08-16       Impact factor: 3.162

10.  Pyruvate dehydrogenase complex from baker's yeast. 2. Molecular structure, dissociation, and implications for the origin of mitochondria.

Authors:  G B Kresze; H Ronft
Journal:  Eur J Biochem       Date:  1981-10
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  15 in total

Review 1.  The 2-oxo acid dehydrogenase complexes: recent advances.

Authors:  S J Yeaman
Journal:  Biochem J       Date:  1989-02-01       Impact factor: 3.857

2.  Survey of amino-terminal proteolytic cleavage sites in mitochondrial precursor proteins: leader peptides cleaved by two matrix proteases share a three-amino acid motif.

Authors:  J P Hendrick; P E Hodges; L E Rosenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

3.  In vivo assembly of yeast mitochondrial alpha-ketoglutarate dehydrogenase complex.

Authors:  B Repetto; A Tzagoloff
Journal:  Mol Cell Biol       Date:  1991-08       Impact factor: 4.272

4.  Isolation of an atypically small lipoamide dehydrogenase involved in the glycine decarboxylase complex from Eubacterium acidaminophilum.

Authors:  W Freudenberg; D Dietrichs; H Lebertz; J R Andreesen
Journal:  J Bacteriol       Date:  1989-03       Impact factor: 3.490

5.  Purification of NADPH-dependent electron-transferring flavoproteins and N-terminal protein sequence data of dihydrolipoamide dehydrogenases from anaerobic, glycine-utilizing bacteria.

Authors:  D Dietrichs; M Meyer; B Schmidt; J R Andreesen
Journal:  J Bacteriol       Date:  1990-04       Impact factor: 3.490

6.  Structure and regulation of KGD2, the structural gene for yeast dihydrolipoyl transsuccinylase.

Authors:  B Repetto; A Tzagoloff
Journal:  Mol Cell Biol       Date:  1990-08       Impact factor: 4.272

7.  Purification and characterization of soybean root nodule ferric leghemoglobin reductase.

Authors:  L Ji; S Wood; M Becana; R V Klucas
Journal:  Plant Physiol       Date:  1991-05       Impact factor: 8.340

8.  Identification of two missense mutations in a dihydrolipoamide dehydrogenase-deficient patient.

Authors:  T C Liu; H Kim; C Arizmendi; A Kitano; M S Patel
Journal:  Proc Natl Acad Sci U S A       Date:  1993-06-01       Impact factor: 11.205

9.  Biochemical and molecular characterization of the Clostridium magnum acetoin dehydrogenase enzyme system.

Authors:  N Krüger; F B Oppermann; H Lorenzl; A Steinbüchel
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

10.  Sequence and organization of genes encoding enzymes involved in pyruvate metabolism in Mycoplasma capricolum.

Authors:  P P Zhu; A Peterkofsky
Journal:  Protein Sci       Date:  1996-08       Impact factor: 6.725
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