Kentaro Ariyoshi1, Tomisato Miura2, Kosuke Kasai3, Valerie Swee Ting Goh3, Yohei Fujishima4, Akifumi Nakata5, Atsushi Takahashi6, Yoshinaka Shimizu7, Hisashi Shinoda7, Hideaki Yamashiro8, Colin Seymour9, Carmel Mothersill9, Mitsuaki A Yoshida2. 1. Integrated Center for Science and Humanities, Fukushima Medical University, Fukushima City, Japan. 2. Department of Radiation Biology, Institute of Radiation Emergency Medicine, Hirosaki University, Hirosaki, Japan. 3. Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences, Hirosaki, Japan. 4. Department of Radiation Biology, Tohoku University School of Medicine, Sendai, Japan. 5. Faculty of Pharmaceutical Sciences, Hokkaido University of Science, Sapporo, Japan. 6. Tohoku University Hospital, Tohoku University, Sendai, Japan. 7. Graduate School of Dentistry, Tohoku University, Sendai, Japan. 8. Graduate School of Science and Technology, Niigata University, Nishiku, Japan. 9. Department of Biology, McMaster University, Hamilton, Canada.
Abstract
PURPOSE: To study the environmental radiation effects of wild animals after the Fukushima Dai-ichi nuclear power plant accident, we assessed effects on hematopoietic progenitor cells (HPCs) in large Japanese field mice (Apodemus speciosus). MATERIALS AND METHODS: A. speciosus were collected from three contaminated sites and control area. The air dose-rates at the control and contaminated areas were 0.96 ± 0.05 μGy/d (Hirosaki), 14.4 ± 2.4 μGy/d (Tanashio), 208.8 ± 31.2 μGy/d (Ide), 470.4 ± 93.6 μGy/d (Omaru), respectively. We investigated possible DNA damage and pro-inflammatory markers in the bone marrow (BM) cells. The colony-forming potential of BM cells was estimated by the number of HPC colony-forming cells. Radiation-induced genomic instability (RIGI) in HPCs was also analyzed by quantifying delayed DNA damage in CFU-GM clones. RESULTS: Although no significant differences in DNA damage and inflammation markers in BM cells from control and contaminated areas, the number of HPC colonies exhibited an inverse correlation with air dose-rate. With regard to RIGI, no significant differences in DNA damage of CFU-GM clones between the mice from the control and the three contaminated areas. CONCLUSIONS: Our study suggests that low dose-rate radiation of more than 200 Gy/d reduced HPCs, possibly eliminating genomically unstable HPCs.
PURPOSE: To study the environmental radiation effects of wild animals after the Fukushima Dai-ichi nuclear power plant accident, we assessed effects on hematopoietic progenitor cells (HPCs) in large Japanese field mice (Apodemus speciosus). MATERIALS AND METHODS: A. speciosus were collected from three contaminated sites and control area. The air dose-rates at the control and contaminated areas were 0.96 ± 0.05 μGy/d (Hirosaki), 14.4 ± 2.4 μGy/d (Tanashio), 208.8 ± 31.2 μGy/d (Ide), 470.4 ± 93.6 μGy/d (Omaru), respectively. We investigated possible DNA damage and pro-inflammatory markers in the bone marrow (BM) cells. The colony-forming potential of BM cells was estimated by the number of HPC colony-forming cells. Radiation-induced genomic instability (RIGI) in HPCs was also analyzed by quantifying delayed DNA damage in CFU-GM clones. RESULTS: Although no significant differences in DNA damage and inflammation markers in BM cells from control and contaminated areas, the number of HPC colonies exhibited an inverse correlation with air dose-rate. With regard to RIGI, no significant differences in DNA damage of CFU-GM clones between the mice from the control and the three contaminated areas. CONCLUSIONS: Our study suggests that low dose-rate radiation of more than 200 Gy/d reduced HPCs, possibly eliminating genomically unstable HPCs.
Authors: Mary Sproull; Joshua Hayes; Hiroko Ishiniwa; Kenji Nanba; Uma Shankavaram; Kevin Camphausen; Thomas E Johnson Journal: Health Phys Date: 2021-12-01 Impact factor: 1.316