Literature DB >> 3278935

Ectoenzymes of the kidney microvillar membrane. Aminopeptidase P is anchored by a glycosyl-phosphatidylinositol moiety.

N M Hooper1, A J Turner.   

Abstract

The mode of membrane anchorage of three kidney microvillar membrane ectoenzymes has been examined. The release of aminopeptidase P (EC 3.4.11.9) from kidney membranes by bacterial phosphatidylinositol-specific phospholipase C (PI-PLC) and the pattern of detergent solubilization of this ectoenzyme implies that it is anchored to the membrane via a covalently attached glycosyl-phosphatidylinositol moiety. As deduced by phase separation in Triton X-114, octyl-glucoside solubilized the amphipathic form of aminopeptidase P, whereas the PI-PLC-released form displayed hydrophilic properties. In contrast, the pattern of detergent solubilization of two microvillar carboxypeptidases and their resistance to release from the membrane by bacterial PI-PLC suggest that these two ectoenzymes are not anchored via phosphatidylinositol.

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Year:  1988        PMID： 3278935     DOI： 10.1016/0014-5793(88)81152-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  27 in total

1.  A survey of membrane peptidases in two human colonic cell lines, Caco-2 and HT-29.

Authors:  S Howell; A J Kenny; A J Turner
Journal:  Biochem J       Date:  1992-06-01       Impact factor: 3.857

2.  The association between glycosylphosphatidylinositol-anchored proteins and heterotrimeric G protein alpha subunits in lymphocytes.

Authors:  K R Solomon; C E Rudd; R W Finberg
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-11       Impact factor: 11.205

Review 3.  Emerging functional roles for the glycosyl-phosphatidylinositol membrane protein anchor.

Authors:  M P Lisanti; E Rodriguez-Boulan; A R Saltiel
Journal:  J Membr Biol       Date:  1990-07       Impact factor: 1.843

4.  Isolation and characterization of two distinct low-density, Triton-insoluble, complexes from porcine lung membranes.

Authors:  E T Parkin; A J Turner; N M Hooper
Journal:  Biochem J       Date:  1996-11-01       Impact factor: 3.857

5.  Insulin stimulates the release of the glycosyl phosphatidylinositol-anchored membrane dipeptidase from 3T3-L1 adipocytes through the action of a phospholipase C.

Authors:  S Movahedi; N M Hooper
Journal:  Biochem J       Date:  1997-09-01       Impact factor: 3.857

6.  Amyloid precursor protein, although partially detergent-insoluble in mouse cerebral cortex, behaves as an atypical lipid raft protein.

Authors:  E T Parkin; A J Turner; N M Hooper
Journal:  Biochem J       Date:  1999-11-15       Impact factor: 3.857

7.  Molecular cloning and expression in COS-1 cells of pig kidney aminopeptidase P.

Authors:  R J Hyde; N M Hooper; A J Turner
Journal:  Biochem J       Date:  1996-10-01       Impact factor: 3.857

8.  Carbonic anhydrase isozymes IV and II in urinary membranes from carbonic anhydrase II-deficient patients.

Authors:  S Sato; X L Zhu; W S Sly
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

9.  Determination of the non-ionic detergent insolubility and phosphoprotein associations of glycosylphosphatidylinositol-anchored proteins expressed on T cells.

Authors:  K R Solomon; M A Mallory; R W Finberg
Journal:  Biochem J       Date:  1998-09-01       Impact factor: 3.857

10.  Expression of an enzymically active glycosylphosphatidylinositol-anchored form of neutral endopeptidase (EC 3.4.24.11) in Cos-1 cells.

Authors:  S Howell; C Lanctôt; G Boileau; P Crine
Journal:  Biochem J       Date:  1994-04-01       Impact factor: 3.857
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