Literature DB >> 32785885

Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs.

Marianna Theresia Traugott1, Wolfgang Hoepler1, Tamara Seitz1, Sebastian Baumgartner1, Mario Karolyi1, Erich Pawelka1, Emanuela Friese1, Stephanie Neuhold1, Hasan Kelani1, Florian Thalhammer2, Alexander Zoufaly1, Hermann Laferl1, Judith Helene Aberle3, Christoph Wenisch1, Elisabeth Puchhammer-Stöckl3, Karin Stiasny3, Stephan Walter Aberle3, Lukas Weseslindtner4.   

Abstract

We report of two cases of progressed COVID-19 with negative PCR tests from nasopharyngeal swabs, in whom diagnosis was made by different antibody assays, including a lateral flow rapid test and multiple commercial ELISAs, finally confirmed by comprehensive serological assays. These cases highlight that commercial ELISAs and even rapid tests might significantly aid the diagnosis of COVID-19, particularly, if a combination of serological assays is used with a specific clinical question, in severely ill patients after seroconversion and when comprehensive serological methods are used for confirmation.

Entities:  

Keywords:  Antibodies; Coronavirus; Immunoassay; PCR negative; Rapid test; SARS

Mesh：

Substances：

Year:  2020        PMID： 32785885      PMCID： PMC7851003          DOI： 10.1007/s15010-020-01497-2

Source DB:  PubMed          Journal:  Infection        ISSN： 0300-8126            Impact factor:   7.455


  16 in total

1.  Evaluation of advanced reverse transcription-PCR assays and an alternative PCR target region for detection of severe acute respiratory syndrome-associated coronavirus.

Authors:  Christian Drosten; Lily-Lily Chiu; Marcus Panning; Hoe Nam Leong; Wolfgang Preiser; John S Tam; Stephan Günther; Stefanie Kramme; Petra Emmerich; Wooi Loon Ng; Herbert Schmitz; Evelyn S C Koay
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

2.  Corrigendum to "Leptospira interrogans infection leads to IL-1β and IL-18 secretion from a human macrophage cell line through reactive oxygen species and cathepsin B mediated-NLRP3 inflammasome activation" [Microbe Infect (2018) 254-260].

Authors:  Shijun Li; Ming Wang; David M Ojcius; Bijun Zhou; Weilin Hu; Ying Liu; Qing Ma; Guangpeng Tan; Dingming Wang; Jie Yan
Journal:  Microbes Infect       Date:  2020-09-26       Impact factor: 2.700

3.  Sensitivity of Chest CT for COVID-19: Comparison to RT-PCR.

Authors:  Yicheng Fang; Huangqi Zhang; Jicheng Xie; Minjie Lin; Lingjun Ying; Peipei Pang; Wenbin Ji
Journal:  Radiology       Date:  2020-02-19       Impact factor: 11.105

4.  Performance of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Assays in Different Stages of Infection: Comparison of Commercial Enzyme-Linked Immunosorbent Assays and Rapid Tests.

Authors:  Marianna Traugott; Stephan Walter Aberle; Judith Helene Aberle; Hannah Griebler; Mario Karolyi; Erich Pawelka; Elisabeth Puchhammer-Stöckl; Alexander Zoufaly; Lukas Weseslindtner
Journal:  J Infect Dis       Date:  2020-07-06       Impact factor: 5.226

5.  Experience of different upper respiratory tract sampling strategies for detection of COVID-19.

Authors:  G Ye; Y Li; M Lu; S Chen; Y Luo; S Wang; Y Wang; X Wang
Journal:  J Hosp Infect       Date:  2020-03-12       Impact factor: 3.926

6.  A Novel Coronavirus from Patients with Pneumonia in China, 2019.

Authors:  Na Zhu; Dingyu Zhang; Wenling Wang; Xingwang Li; Bo Yang; Jingdong Song; Xiang Zhao; Baoying Huang; Weifeng Shi; Roujian Lu; Peihua Niu; Faxian Zhan; Xuejun Ma; Dayan Wang; Wenbo Xu; Guizhen Wu; George F Gao; Wenjie Tan
Journal:  N Engl J Med       Date:  2020-01-24       Impact factor: 91.245

7.  Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study.

Authors:  J S M Peiris; C M Chu; V C C Cheng; K S Chan; I F N Hung; L L M Poon; K I Law; B S F Tang; T Y W Hon; C S Chan; K H Chan; J S C Ng; B J Zheng; W L Ng; R W M Lai; Y Guan; K Y Yuen
Journal:  Lancet       Date:  2003-05-24       Impact factor: 79.321

8.  Effect of changing case definitions for COVID-19 on the epidemic curve and transmission parameters in mainland China: a modelling study.

Authors:  Tim K Tsang; Peng Wu; Yun Lin; Eric H Y Lau; Gabriel M Leung; Benjamin J Cowling
Journal:  Lancet Public Health       Date:  2020-04-21

9.  SARS-CoV-2 shedding and seroconversion among passengers quarantined after disembarking a cruise ship: a case series.

Authors:  Ivan Fan-Ngai Hung; Vincent Chi-Chung Cheng; Xin Li; Anthony Raymond Tam; Derek Ling-Lung Hung; Kelvin Hei-Yeung Chiu; Cyril Chik-Yan Yip; Jian-Piao Cai; Deborah Tip-Yin Ho; Shuk-Ching Wong; Sally Sau-Man Leung; Man-Yee Chu; Milky Oi-Yan Tang; Jonathan Hon-Kwan Chen; Rosana Wing-Shan Poon; Agnes Yim-Fong Fung; Ricky Ruiqi Zhang; Erica Yuen-Wing Yan; Lin-Lei Chen; Charlotte Yee-Ki Choi; Kit-Hang Leung; Tom Wai-Hin Chung; Sonia Hiu-Yin Lam; Tina Poy-Wing Lam; Jasper Fuk-Woo Chan; Kwok-Hung Chan; Tak-Chiu Wu; Pak-Leung Ho; Johnny Wai-Man Chan; Chak-Sing Lau; Kelvin Kai-Wang To; Kwok-Yung Yuen
Journal:  Lancet Infect Dis       Date:  2020-06-12       Impact factor: 25.071

10.  Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020.

Authors:  Rui Liu; Huan Han; Fang Liu; Zhihua Lv; Kailang Wu; Yingle Liu; Yong Feng; Chengliang Zhu
Journal:  Clin Chim Acta       Date:  2020-03-07       Impact factor: 3.786
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  5 in total

1.  SARS-CoV-2-mRNA Booster Vaccination Reverses Non-Responsiveness and Early Antibody Waning in Immunocompromised Patients - A Phase Four Study Comparing Immune Responses in Patients With Solid Cancers, Multiple Myeloma and Inflammatory Bowel Disease.

Authors:  Angelika Wagner; Erika Garner-Spitzer; Anna-Margarita Schötta; Maria Orola; Andrea Wessely; Ines Zwazl; Anna Ohradanova-Repic; Lukas Weseslindtner; Gabor Tajti; Laura Gebetsberger; Bernhard Kratzer; Elena Tomosel; Maximilian Kutschera; Selma Tobudic; Winfried F Pickl; Michael Kundi; Hannes Stockinger; Gottfried Novacek; Walter Reinisch; Christoph Zielinski; Ursula Wiedermann
Journal:  Front Immunol       Date:  2022-05-12       Impact factor: 8.786

2.  Lessons from low seroprevalence of SARS-CoV-2 antibodies in schoolchildren: A cross-sectional study.

Authors:  Zsolt Szépfalusi; Klara Schmidthaler; Justyna Sieber; Sonja Kopanja; Florian Götzinger; Anja Schoof; Jakub Hoz; Birgit Willinger; Athanasios Makristathis; Lukas Weseslindtner; Karin Stiasny; Barbara Bohle; Pavla Krotka; Alexandra Graf; Thomas Frischer
Journal:  Pediatr Allergy Immunol       Date:  2021-02-15       Impact factor: 5.464

3.  A Longitudinal Seroprevalence Study Evaluating Infection Control and Prevention Strategies at a Large Tertiary Care Center with Low COVID-19 Incidence.

Authors:  Lorenz Schubert; Robert Strassl; Heinz Burgmann; Gabriella Dvorak; Matthias Karer; Michael Kundi; Manuel Kussmann; Heimo Lagler; Felix Lötsch; Christopher Milacek; Markus Obermueller; Zoe Oesterreicher; Christoph Steininger; Karin Stiasny; Florian Thalhammer; Ludwig Traby; Zoltan Vass; Matthias Gerhard Vossen; Lukas Weseslindtner; Stefan Winkler; Selma Tobudic
Journal:  Int J Environ Res Public Health       Date:  2021-04-15       Impact factor: 3.390

4.  Screening and Confirmatory Testing for SARS-CoV-2 Antibodies: Comparison of Health and Non-Health Workers in a Nationwide Healthcare Organization in Central Europe.

Authors:  Johann Bartko; Sonja Zehetmayer; Lukas Weseslindtner; Karin Stiasny; Andrea Schloegl; Ernst Forjan; Elisabeth Zwettler; Andreas Krauter; Felix Keil; Nazanin Sédille-Mostafaie
Journal:  J Clin Med       Date:  2021-04-28       Impact factor: 4.241

Review 5.  [Diagnosis and treatment of COVID-19 in intensive care units].

Authors:  Wolfgang Hoepler; Marianna Traugott; Alexander Zoufaly; Martina Schatzl; Julian Hind; Christoph Wenisch; Stephanie Neuhold
Journal:  Med Klin Intensivmed Notfmed       Date:  2022-03-28       Impact factor: 1.552
  5 in total

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