Jui-Feng Chang1, Huei-Chun Liu1, H H Chen1, Wen-Ping Chen2, Jian-Lin Juang3, Pei-Ning Wang4,5,6, Shieh-Yueh Yang7,8. 1. MagQu Co., Ltd., New Taipei City, Taiwan. 2. MagQu LLC, Surprise, Arizona, USA. 3. Bio-Check Laboratories Ltd., New Taipei City, Taiwan. 4. Division of General Neurology, Department of Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan. 5. Department of Neurology, School of Medicine, National Yang-Ming University, Taipei, Taiwan. 6. Brain Research Center, National Yang-Ming University, Taipei, Taiwan. 7. MagQu Co., Ltd., New Taipei City, Taiwan, syyang@magqu.com. 8. MagQu LLC, Surprise, Arizona, USA, syyang@magqu.com.
Abstract
BACKGROUND: The stability of proteins in the collecting tubes after blood draw is critical to the measured concentrations of the proteins. Although the guidelines issued by the Clinical and Laboratory Standards Institute (CLSI) suggest centrifugation should take place within 2 h of drawing blood, it is very difficult to follow these guidelines in hospitals or clinics. It is necessary to study the effect of times to blood processing on the stability of the proteins of interest. METHODS: In this work, the plasma proteins of interest were those relevant to dementia, such as amyloid β 1-40 (Aβ1-40), Aβ1-42, Tau protein (Tau), and α-synuclein. The times to blood processing after blood draw ranged from 0.5 to 8 h. The storage temperatures of blood were room temperature (approx. 25°C) and 30°C. After storage, blood samples were centrifuged at room temperature to obtain plasma samples. Ultrasensitive immunomagnetic reduction was applied to assay these proteins in the plasma. RESULTS: The levels of plasma Aβ1-40, Tau, and α-synuclein did not significantly change until 8 h after blood draw when stored at room temperature. Plasma Aβ1-42 levels did not change significantly after 8 h of storage at room temperature before blood processing. Higher storage temperatures, such as 30°C, for blood samples accelerated the significant variations in the measured concentrations of Aβ1-40, Tau, and α-synuclein in plasma. CONCLUSION: According to these results, for clinical practice, it is suggested that blood samples be stored at room temperature for no longer than 4.5 h after blood draw until centrifugation for the assay of dementia biomarkers in plasma.
BACKGROUND: The stability of proteins in the collecting tubes after blood draw is critical to the measured concentrations of the proteins. Although the guidelines issued by the Clinical and Laboratory Standards Institute (CLSI) suggest centrifugation should take place within 2 h of drawing blood, it is very difficult to follow these guidelines in hospitals or clinics. It is necessary to study the effect of times to blood processing on the stability of the proteins of interest. METHODS: In this work, the plasma proteins of interest were those relevant to dementia, such as amyloid β 1-40 (Aβ1-40), Aβ1-42, Tau protein (Tau), and α-synuclein. The times to blood processing after blood draw ranged from 0.5 to 8 h. The storage temperatures of blood were room temperature (approx. 25°C) and 30°C. After storage, blood samples were centrifuged at room temperature to obtain plasma samples. Ultrasensitive immunomagnetic reduction was applied to assay these proteins in the plasma. RESULTS: The levels of plasma Aβ1-40, Tau, and α-synuclein did not significantly change until 8 h after blood draw when stored at room temperature. Plasma Aβ1-42 levels did not change significantly after 8 h of storage at room temperature before blood processing. Higher storage temperatures, such as 30°C, for blood samples accelerated the significant variations in the measured concentrations of Aβ1-40, Tau, and α-synuclein in plasma. CONCLUSION: According to these results, for clinical practice, it is suggested that blood samples be stored at room temperature for no longer than 4.5 h after blood draw until centrifugation for the assay of dementia biomarkers in plasma.
Authors: Inge M W Verberk; Els O Misdorp; Jannet Koelewijn; Andrew J Ball; Kaj Blennow; Jeffrey L Dage; Noelia Fandos; Oskar Hansson; Christophe Hirtz; Shorena Janelidze; Sungmin Kang; Kristopher Kirmess; Jana Kindermans; Ryan Lee; Matthew R Meyer; Dandan Shan; Leslie M Shaw; Teresa Waligorska; Tim West; Henrik Zetterberg; Rebecca M Edelmayer; Charlotte E Teunissen Journal: Alzheimers Dement Date: 2021-11-29 Impact factor: 16.655