Hajer Ben Khadhra1, Françoise Rose-Robert2, Yves Edouard Herpe3, Henri Sevestre4, Gabriel Choukroun5, Luc Catherine6, Carole Amant7, Fabien Saint8,9,10. 1. Public Health and General Cancer Register de La Somme, Jules Verne University, CHU Amiens, 80000, Amiens, France. 2. Laboratory of Biochemistry, Jules Verne University, CHU Amiens, 80000, Amiens, France. 3. BRC (Biobanque de Picardie), Jules Verne University, CHU Amiens, 80000, Amiens, France. 4. Pathology, Jules Verne University, CHU Amiens, 80000, Amiens, France. 5. Nephrology, Jules Verne University, CHU Amiens, 80000, Amiens, France. 6. Association "Juste Un Petit Rein", Jules Verne University, CHU Amiens, 80000, Amiens, France. 7. EPROAD EA 4669, Jules Verne University, CHU Amiens, 80000, Amiens, France. 8. Departments of Urology-Transplantation, Jules Verne University, CHU Amiens, 80000, Amiens, France. Fabiensaint@hotmail.com. 9. EPROAD EA 4669, Jules Verne University, CHU Amiens, 80000, Amiens, France. Fabiensaint@hotmail.com. 10. BRC (Biobanque de Picardie), Jules Verne University, CHU Amiens, 80000, Amiens, France. Fabiensaint@hotmail.com.
Abstract
INTRODUCTION: Biomarkers for the diagnosis and monitoring treatment response of kidney cancer are urgently needed. Neutrophil gelatinase-associated lipocalin (NGAL) is a relevant urinary biomarker for the diagnosis of a wide variety of acute and chronic kidney diseases. Its potential utility as a prognostic marker of kidney cancer is largely unknown and, therefore, was the subject of this investigation. MATERIALS AND METHODS: A retrospective study was done on 50 kidney tumor patients (urine samples prospectively collected before nephrectomy between 2004 and 2012, stored at Biobank Resource Center). The specificity, sensitivity and the predictive value of NGAL were determined for progression-free and disease-specific survival after nephrectomy in renal cell carcinoma (particularly, the clear cell renal cell carcinoma (ccRCC)). Urinary NGAL concentration (u-NGAL) was determined by CMIA technique (ARCHITECT® urine NGAL essay/ABBOTT®). RESULTS: Out of the 50 kidney tumor patients, 40 had clear cell carcinoma with a median u-NGAL excretion of 1.4 (IQR: 5.76) ng/mg urinary creatinine (Ucr). u-NGAL was correlated to tumor stage (p = 0.005), and Fuhrman grade (p = 0.0002). Multivariate Cox regression analysis showed a significant association between u-NGAL excretion and clear cell renal cell carcinoma progression free survival and disease specific survival (p = 0.002; p = 0.0001). CONCLUSIONS: Urinary NGAL was significantly associated with the stage and the grade of kidney cancer. u-NGAL excretion could be considered as a potential biomarker to identify ccRCC patients with the more pejorative outcomes.
INTRODUCTION: Biomarkers for the diagnosis and monitoring treatment response of kidney cancer are urgently needed. Neutrophil gelatinase-associated lipocalin (NGAL) is a relevant urinary biomarker for the diagnosis of a wide variety of acute and chronic kidney diseases. Its potential utility as a prognostic marker of kidney cancer is largely unknown and, therefore, was the subject of this investigation. MATERIALS AND METHODS: A retrospective study was done on 50 kidney tumorpatients (urine samples prospectively collected before nephrectomy between 2004 and 2012, stored at Biobank Resource Center). The specificity, sensitivity and the predictive value of NGAL were determined for progression-free and disease-specific survival after nephrectomy in renal cell carcinoma (particularly, the clear cell renal cell carcinoma (ccRCC)). Urinary NGAL concentration (u-NGAL) was determined by CMIA technique (ARCHITECT® urine NGAL essay/ABBOTT®). RESULTS: Out of the 50 kidney tumorpatients, 40 had clear cell carcinoma with a median u-NGAL excretion of 1.4 (IQR: 5.76) ng/mg urinary creatinine (Ucr). u-NGAL was correlated to tumor stage (p = 0.005), and Fuhrman grade (p = 0.0002). Multivariate Cox regression analysis showed a significant association between u-NGAL excretion and clear cell renal cell carcinoma progression free survival and disease specific survival (p = 0.002; p = 0.0001). CONCLUSIONS: Urinary NGAL was significantly associated with the stage and the grade of kidney cancer. u-NGAL excretion could be considered as a potential biomarker to identify ccRCC patients with the more pejorative outcomes.
Authors: C Perrin; J-J Patard; F Jouan; N Collet; S Théoleyre; J Edeline; S Zerrouki; B Laguerre; M-A Bellaud-Roturaud; N Rioux-Leclercq; C Vigneau Journal: Prog Urol Date: 2011-09-03 Impact factor: 0.915
Authors: G Kovacs; M Akhtar; B J Beckwith; P Bugert; C S Cooper; B Delahunt; J N Eble; S Fleming; B Ljungberg; L J Medeiros; H Moch; V E Reuter; E Ritz; G Roos; D Schmidt; J R Srigley; S Störkel; E van den Berg; B Zbar Journal: J Pathol Date: 1997-10 Impact factor: 7.996