| Literature DB >> 32778832 |
Cara R Schiavon1,2, Tong Zhang1, Bing Zhao3, Andrew S Moore4, Pauline Wales1,2, Leonardo R Andrade1, Melissa Wu1, Tsung-Chang Sung5, Yelena Dayn5, Jasmine W Feng6, Omar A Quintero6, Gerald S Shadel2, Robert Grosse3, Uri Manor7.
Abstract
The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are challenging to detect with fluorescence microscopy, making it difficult to determine whether actin filaments are directly associated with specific membranes. To address these limitations, we developed fluorescent-protein-tagged actin nanobodies, termed 'actin chromobodies' (ACs), targeted to organelle membranes to enable high-resolution imaging of sub-organellar actin dynamics.Entities:
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Year: 2020 PMID： 32778832 PMCID： PMC7746311 DOI： 10.1038/s41592-020-0926-5
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547